YKL-40 and matrix metalloproteinases as potential biomarkers of inflammation and fibrosis in the development of bronchiolitis obliterans syndrome

E A Kastelijn, C H M van Moorsel, H J T Ruven, N M Korthagen, J M Kwakkel-van Erp, E A van de Graaf, P Zanen, D A van Kessel, J C Grutters

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

BACKGROUND AND OBJECTIVE: The development of bronchiolitis obliterans syndrome (BOS) after lung transplantation is characterized by inflammation, remodeling and fibrosis. Both YKL-40 and matrix metalloproteinase (MMP)-9 have shown to be involved in these processes. We measured serial YKL-40 and MMP-9 serum levels in lung transplant recipients and assessed their usefulness as biomarker for BOS. Furthermore, we investigate the relationship between these two potential biomarkers of BOS and MMP-7.

DESIGN: Ten patients with BOS (BOS(pos)) and 10 matched patients without BOS (BOS(neg)) were included. Serial serum samples were collected after lung transplantation and prior to BOS. YKL-40, MMP-9 and MMP-7 serum levels were determined by ELISA.

RESULTS: The median concentrations of YKL-40 did not differ between BOS(pos) and BOS(neg) patients (p > 0.05). The median concentration of MMP-9 in BOS(pos) patients was significantly higher than in BOS(neg) patients (p < 0.0001). For MMP-9 as possible risk factor for BOS, a cut off value of 145 ng/ml has a sensitivity of 90% and a negative predictive value of 83%. Longitudinal analysis of YKL-40 and MMP-9 serum levels from the early post-transplant period onwards did not reveal a significant trend in time in both serum levels preceding BOS. In BOS(neg) patients MMP-9 showed an inverse relationship with MMP-7, that was absent in BOS(pos) patients.

CONCLUSIONS: From the moment of transplantation onwards, patients who eventually developed BOS had significantly increased MMP-9 serum levels in comparison with patients who did not develop BOS. Therefore, increased MMP-9 serum levels might be useful as risk factor for BOS.

Original languageEnglish
Pages (from-to)28-35
Number of pages8
JournalSarcoidosis, Vasculitis, and Diffuse Lung Diseases
Volume30
Issue number1
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Adipokines
  • Adult
  • Biological Markers
  • Bronchiolitis Obliterans
  • Female
  • Humans
  • Lectins
  • Lung Diseases
  • Lung Transplantation
  • Male
  • Matrix Metalloproteinase 7
  • Matrix Metalloproteinase 9
  • Middle Aged
  • Risk Factors

Fingerprint

Dive into the research topics of 'YKL-40 and matrix metalloproteinases as potential biomarkers of inflammation and fibrosis in the development of bronchiolitis obliterans syndrome'. Together they form a unique fingerprint.

Cite this