Xylitol production from plant biomass by Aspergillus niger through metabolic engineering

Jiali Meng; Tania Chroumpi; Miia R. Mäkelä; Ronald P. de Vries

doi:10.1016/j.biortech.2021.126199

Xylitol production from plant biomass by Aspergillus niger through metabolic engineering

Jiali Meng
, Tania Chroumpi
, Miia R. Mäkelä
, Ronald P. de Vries^*

^*Corresponding author for this work

Molecular Plant Physiology

Utrecht University
University of Helsinki

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Xylitol is widely used in the food and pharmaceutical industries as a valuable commodity product. Biotechnological production of xylitol from lignocellulosic biomass by microorganisms is a promising alternative option to chemical synthesis or bioconversion from D-xylose. In this study, four metabolic mutants of Aspergillus niger were constructed and evaluated for xylitol accumulation from D-xylose and lignocellulosic biomass. All mutants had strongly increased xylitol production from pure D-xylose, beechwood xylan, wheat bran and cotton seed hulls compared to the reference strain, but not from several other feed stocks. The triple mutant ΔladAΔxdhAΔsdhA showed the best performance in xylitol production from wheat bran and cotton seed hulls. This study demonstrated the large potential of A. niger for xylitol production directly from lignocellulosic biomass by metabolic engineering.

Original language	English
Article number	126199
Pages (from-to)	1-9
Journal	Bioresource Technology
Volume	344
Issue number	Part A
DOIs	https://doi.org/10.1016/j.biortech.2021.126199
Publication status	Published - Jan 2022

Bibliographical note

Funding Information:
This work was supported by the China Scholarship Council (CSC student number: CSC201907720027 to JM) and the Academy of Finland (grant no. 308,284 to MRM).

Publisher Copyright:
© 2021 The Author(s)

Funding

This work was supported by the China Scholarship Council (CSC student number: CSC201907720027 to JM) and the Academy of Finland (grant no. 308,284 to MRM).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Aspergillus niger
Lignocellulosic biomass
Metabolic engineering
Pentose catabolic pathway
Xylitol production

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1-s2.0-S0960852421015418-mainFinal published version, 2.84 MBLicence: CC BY

Cite this

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title = "Xylitol production from plant biomass by Aspergillus niger through metabolic engineering",

abstract = "Xylitol is widely used in the food and pharmaceutical industries as a valuable commodity product. Biotechnological production of xylitol from lignocellulosic biomass by microorganisms is a promising alternative option to chemical synthesis or bioconversion from D-xylose. In this study, four metabolic mutants of Aspergillus niger were constructed and evaluated for xylitol accumulation from D-xylose and lignocellulosic biomass. All mutants had strongly increased xylitol production from pure D-xylose, beechwood xylan, wheat bran and cotton seed hulls compared to the reference strain, but not from several other feed stocks. The triple mutant ΔladAΔxdhAΔsdhA showed the best performance in xylitol production from wheat bran and cotton seed hulls. This study demonstrated the large potential of A. niger for xylitol production directly from lignocellulosic biomass by metabolic engineering.",

keywords = "Aspergillus niger, Lignocellulosic biomass, Metabolic engineering, Pentose catabolic pathway, Xylitol production",

author = "Jiali Meng and Tania Chroumpi and M{\"a}kel{\"a}, \{Miia R.\} and \{de Vries\}, \{Ronald P.\}",

note = "Funding Information: This work was supported by the China Scholarship Council (CSC student number: CSC201907720027 to JM) and the Academy of Finland (grant no. 308,284 to MRM). Publisher Copyright: {\textcopyright} 2021 The Author(s)",

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AU - Chroumpi, Tania

AU - Mäkelä, Miia R.

AU - de Vries, Ronald P.

N1 - Funding Information: This work was supported by the China Scholarship Council (CSC student number: CSC201907720027 to JM) and the Academy of Finland (grant no. 308,284 to MRM). Publisher Copyright: © 2021 The Author(s)

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N2 - Xylitol is widely used in the food and pharmaceutical industries as a valuable commodity product. Biotechnological production of xylitol from lignocellulosic biomass by microorganisms is a promising alternative option to chemical synthesis or bioconversion from D-xylose. In this study, four metabolic mutants of Aspergillus niger were constructed and evaluated for xylitol accumulation from D-xylose and lignocellulosic biomass. All mutants had strongly increased xylitol production from pure D-xylose, beechwood xylan, wheat bran and cotton seed hulls compared to the reference strain, but not from several other feed stocks. The triple mutant ΔladAΔxdhAΔsdhA showed the best performance in xylitol production from wheat bran and cotton seed hulls. This study demonstrated the large potential of A. niger for xylitol production directly from lignocellulosic biomass by metabolic engineering.

AB - Xylitol is widely used in the food and pharmaceutical industries as a valuable commodity product. Biotechnological production of xylitol from lignocellulosic biomass by microorganisms is a promising alternative option to chemical synthesis or bioconversion from D-xylose. In this study, four metabolic mutants of Aspergillus niger were constructed and evaluated for xylitol accumulation from D-xylose and lignocellulosic biomass. All mutants had strongly increased xylitol production from pure D-xylose, beechwood xylan, wheat bran and cotton seed hulls compared to the reference strain, but not from several other feed stocks. The triple mutant ΔladAΔxdhAΔsdhA showed the best performance in xylitol production from wheat bran and cotton seed hulls. This study demonstrated the large potential of A. niger for xylitol production directly from lignocellulosic biomass by metabolic engineering.

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KW - Pentose catabolic pathway

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Xylitol production from plant biomass by Aspergillus niger through metabolic engineering

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

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