Within-farm dynamics of ESBL/AmpC-producing Escherichia coli in veal calves: a longitudinal approach

Joost Hordijk; Dik J Mevius; Arie Kant; Marian E H Bos; Haitske Graveland; Angela B Bosman; Cedric M Hartskeerl; Dick J J Heederik; Jaap A Wagenaar

doi:10.1093/jac/dkt219

Within-farm dynamics of ESBL/AmpC-producing Escherichia coli in veal calves: a longitudinal approach

Joost Hordijk, Dik J Mevius, Arie Kant, Marian E H Bos, Haitske Graveland, Angela B Bosman, Cedric M Hartskeerl, Dick J J Heederik, Jaap A Wagenaar

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

OBJECTIVES: To assess the within-farm dynamics of extended-spectrum β-lactamase (ESBL)/AmpC-producing Escherichia coli in veal calves.

METHODS: Three veal-calf fattening farms were screened. Faecal samples from all calves within a compartment (109-150 per farm) were taken upon arrival on the farm (T0) and after 3, 6, 8 and 10 weeks (T3-T10). ESBL/AmpC genes were characterized by PCR and sequencing. Plasmids were characterized by transformation, PCR-based replicon typing and plasmid multilocus sequence typing (MLST). E. coli genotypes were analysed by MLST.

RESULTS: At T0 the prevalence of ESBL/AmpC-producing E. coli ranged from 18% to 26%. These were predominantly isolates carrying blaCTX-M-1 and blaCTX-M-15 genes, located on various plasmids and E. coli sequence types (STs). Farm 1 was negative for ESBL/AmpC-producing E. coli after T0. Farm 2 showed an increase up to 37% at T3, which subsequently decreased gradually to 0% at T10. The presence from T3 to T10 on farm 2 was mainly caused by the clonal spread of a multiresistant E. coli ST57 harbouring blaCTX-M-14 on an IncF F2:A-:B- plasmid. Farm 3 showed a gradual decrease in prevalence to 1.4% at T10, with a relative increase of the identical clonal variant as shown for farm 2. A second clonal variant found in farm 3 was a multiresistant E. coli ST10 harbouring blaCTX-M-14 on an IncK plasmid.

CONCLUSIONS: The prevalence of ESBL/AmpC-producing E. coli decreased over time. A clonal spread was observed on farm 2 and farm 3, illustrative of the complex dynamics probably associated with the use of antimicrobials.

Original language	English
Pages (from-to)	2468-2476
Number of pages	9
Journal	Journal of Antimicrobial Chemotherapy
Volume	68
Issue number	11
DOIs	https://doi.org/10.1093/jac/dkt219
Publication status	Published - Nov 2013

Keywords

Animals
Animals, Domestic
Cattle
DNA, Bacterial
Escherichia coli
Escherichia coli Infections
Feces
Genotype
Molecular Epidemiology
Multilocus Sequence Typing
Plasmids
Polymerase Chain Reaction
Prevalence
Transformation, Bacterial
beta-Lactamases

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title = "Within-farm dynamics of ESBL/AmpC-producing Escherichia coli in veal calves: a longitudinal approach",

abstract = "OBJECTIVES: To assess the within-farm dynamics of extended-spectrum β-lactamase (ESBL)/AmpC-producing Escherichia coli in veal calves.METHODS: Three veal-calf fattening farms were screened. Faecal samples from all calves within a compartment (109-150 per farm) were taken upon arrival on the farm (T0) and after 3, 6, 8 and 10 weeks (T3-T10). ESBL/AmpC genes were characterized by PCR and sequencing. Plasmids were characterized by transformation, PCR-based replicon typing and plasmid multilocus sequence typing (MLST). E. coli genotypes were analysed by MLST.RESULTS: At T0 the prevalence of ESBL/AmpC-producing E. coli ranged from 18% to 26%. These were predominantly isolates carrying blaCTX-M-1 and blaCTX-M-15 genes, located on various plasmids and E. coli sequence types (STs). Farm 1 was negative for ESBL/AmpC-producing E. coli after T0. Farm 2 showed an increase up to 37% at T3, which subsequently decreased gradually to 0% at T10. The presence from T3 to T10 on farm 2 was mainly caused by the clonal spread of a multiresistant E. coli ST57 harbouring blaCTX-M-14 on an IncF F2:A-:B- plasmid. Farm 3 showed a gradual decrease in prevalence to 1.4% at T10, with a relative increase of the identical clonal variant as shown for farm 2. A second clonal variant found in farm 3 was a multiresistant E. coli ST10 harbouring blaCTX-M-14 on an IncK plasmid.CONCLUSIONS: The prevalence of ESBL/AmpC-producing E. coli decreased over time. A clonal spread was observed on farm 2 and farm 3, illustrative of the complex dynamics probably associated with the use of antimicrobials.",

keywords = "Animals, Animals, Domestic, Cattle, DNA, Bacterial, Escherichia coli, Escherichia coli Infections, Feces, Genotype, Molecular Epidemiology, Multilocus Sequence Typing, Plasmids, Polymerase Chain Reaction, Prevalence, Transformation, Bacterial, beta-Lactamases",

author = "Joost Hordijk and Mevius, {Dik J} and Arie Kant and Bos, {Marian E H} and Haitske Graveland and Bosman, {Angela B} and Hartskeerl, {Cedric M} and Heederik, {Dick J J} and Wagenaar, {Jaap A}",

year = "2013",

month = nov,

doi = "10.1093/jac/dkt219",

language = "English",

volume = "68",

pages = "2468--2476",

journal = "Journal of Antimicrobial Chemotherapy",

issn = "0305-7453",

publisher = "Oxford University Press",

number = "11",

}

TY - JOUR

T1 - Within-farm dynamics of ESBL/AmpC-producing Escherichia coli in veal calves

T2 - a longitudinal approach

AU - Hordijk, Joost

AU - Mevius, Dik J

AU - Kant, Arie

AU - Bos, Marian E H

AU - Graveland, Haitske

AU - Bosman, Angela B

AU - Hartskeerl, Cedric M

AU - Heederik, Dick J J

AU - Wagenaar, Jaap A

PY - 2013/11

Y1 - 2013/11

N2 - OBJECTIVES: To assess the within-farm dynamics of extended-spectrum β-lactamase (ESBL)/AmpC-producing Escherichia coli in veal calves.METHODS: Three veal-calf fattening farms were screened. Faecal samples from all calves within a compartment (109-150 per farm) were taken upon arrival on the farm (T0) and after 3, 6, 8 and 10 weeks (T3-T10). ESBL/AmpC genes were characterized by PCR and sequencing. Plasmids were characterized by transformation, PCR-based replicon typing and plasmid multilocus sequence typing (MLST). E. coli genotypes were analysed by MLST.RESULTS: At T0 the prevalence of ESBL/AmpC-producing E. coli ranged from 18% to 26%. These were predominantly isolates carrying blaCTX-M-1 and blaCTX-M-15 genes, located on various plasmids and E. coli sequence types (STs). Farm 1 was negative for ESBL/AmpC-producing E. coli after T0. Farm 2 showed an increase up to 37% at T3, which subsequently decreased gradually to 0% at T10. The presence from T3 to T10 on farm 2 was mainly caused by the clonal spread of a multiresistant E. coli ST57 harbouring blaCTX-M-14 on an IncF F2:A-:B- plasmid. Farm 3 showed a gradual decrease in prevalence to 1.4% at T10, with a relative increase of the identical clonal variant as shown for farm 2. A second clonal variant found in farm 3 was a multiresistant E. coli ST10 harbouring blaCTX-M-14 on an IncK plasmid.CONCLUSIONS: The prevalence of ESBL/AmpC-producing E. coli decreased over time. A clonal spread was observed on farm 2 and farm 3, illustrative of the complex dynamics probably associated with the use of antimicrobials.

AB - OBJECTIVES: To assess the within-farm dynamics of extended-spectrum β-lactamase (ESBL)/AmpC-producing Escherichia coli in veal calves.METHODS: Three veal-calf fattening farms were screened. Faecal samples from all calves within a compartment (109-150 per farm) were taken upon arrival on the farm (T0) and after 3, 6, 8 and 10 weeks (T3-T10). ESBL/AmpC genes were characterized by PCR and sequencing. Plasmids were characterized by transformation, PCR-based replicon typing and plasmid multilocus sequence typing (MLST). E. coli genotypes were analysed by MLST.RESULTS: At T0 the prevalence of ESBL/AmpC-producing E. coli ranged from 18% to 26%. These were predominantly isolates carrying blaCTX-M-1 and blaCTX-M-15 genes, located on various plasmids and E. coli sequence types (STs). Farm 1 was negative for ESBL/AmpC-producing E. coli after T0. Farm 2 showed an increase up to 37% at T3, which subsequently decreased gradually to 0% at T10. The presence from T3 to T10 on farm 2 was mainly caused by the clonal spread of a multiresistant E. coli ST57 harbouring blaCTX-M-14 on an IncF F2:A-:B- plasmid. Farm 3 showed a gradual decrease in prevalence to 1.4% at T10, with a relative increase of the identical clonal variant as shown for farm 2. A second clonal variant found in farm 3 was a multiresistant E. coli ST10 harbouring blaCTX-M-14 on an IncK plasmid.CONCLUSIONS: The prevalence of ESBL/AmpC-producing E. coli decreased over time. A clonal spread was observed on farm 2 and farm 3, illustrative of the complex dynamics probably associated with the use of antimicrobials.

KW - Animals

KW - Animals, Domestic

KW - Cattle

KW - DNA, Bacterial

KW - Escherichia coli

KW - Escherichia coli Infections

KW - Feces

KW - Genotype

KW - Molecular Epidemiology

KW - Multilocus Sequence Typing

KW - Plasmids

KW - Polymerase Chain Reaction

KW - Prevalence

KW - Transformation, Bacterial

KW - beta-Lactamases

U2 - 10.1093/jac/dkt219

DO - 10.1093/jac/dkt219

M3 - Article

C2 - 23759504

SN - 0305-7453

VL - 68

SP - 2468

EP - 2476

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 11

ER -

Within-farm dynamics of ESBL/AmpC-producing Escherichia coli in veal calves: a longitudinal approach

Abstract

Keywords

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