Abstract
Enveloped viruses carry one or multiple proteins with receptor-binding functionalities. Functional receptors can be glycans, proteinaceous, or both; therefore, recombinant protein approaches are instrumental in attaining new insights regarding viral envelope protein receptor-binding properties. Visualizing and measuring receptor binding typically entails antibody detection or direct labeling, whereas direct fluorescent fusions are attractive tools in molecular biology. Here, we report a suite of distinct fluorescent fusions, both N- and C-terminal, for influenza A virus hemagglutinins and SARS-CoV-2 spike RBD. The proteins contained three or six fluorescent protein barrels and were applied directly to cells to assess receptor binding properties.
Original language | English |
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Article number | e4974 |
Number of pages | 13 |
Journal | Protein science |
Volume | 33 |
Issue number | 4 |
DOIs | |
Publication status | Published - Apr 2024 |
Bibliographical note
Publisher Copyright:© 2024 The Authors. Protein Science published by Wiley Periodicals LLC on behalf of The Protein Society.
Funding
R.P.dV is a recipient of an ERC Starting Grant from the European Commission (802780). We thank the Netherlands Organization for Scientific Research (NWO) for the Rubicon Grant 45219118 to A.T.d.l.P.
Funders | Funder number |
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European Commission | 802780 |
Nederlandse Organisatie voor Wetenschappelijk Onderzoek | 45219118 |
Keywords
- attachment protein
- GFP
- hemagglutinin
- influenza a virus
- multivalency
- receptor-binding
- SARS-CoV-2