Validation of transferability of DBA derivatization and LC-MS/MS determination method for isocyanates via an interlaboratory comparison.

I. Bobeldijk; D. Karlsson; A. Pronk; J. Gonsalves; M. Hekman; D. van de Lagemaat; L. Preller; D. Heederik; G. Skarping

doi:10.1093/annhyg/men057

Validation of transferability of DBA derivatization and LC-MS/MS determination method for isocyanates via an interlaboratory comparison.

I. Bobeldijk, D. Karlsson, A. Pronk, J. Gonsalves, M. Hekman, D. van de Lagemaat, L. Preller, D. Heederik, G. Skarping

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Research output: Contribution to journal › Article › Academic › peer-review

Abstract

An adapted method for the quantitative determination of isocyanates in air was implemented and validated in-house. The method was based on air sampling using an impinger flask containing di-n-butylamine (DBA) in toluene and a glass fibre filter in series. The DBA derivatives were determined using liquid chromatography and tandem mass spectrometry. Studied isocyanates were isophorone diisocyanate, isocyanic acid (ICA), methyl isocyanate, ethyl isocyanate, propyl isocyanate, hexamethylene diisocyanate (HDI), 2,6- and 2,4-toluene diisocyanate, 4,4'-methylene diphenyl diisocyanate (MDI), phenyl isocyanate (PhI), MDI oligomers and different HDI adducts. Monitoring of selected reactions resulted in quantifications with correlation coefficients >0.995, within-batch relative standard deviation (RSD) of repeatability was 500 samples.

Original language	Undefined/Unknown
Pages (from-to)	757-763
Number of pages	6
Journal	Annals of Occupational Hygiene
Volume	52
Issue number	8
DOIs	https://doi.org/10.1093/annhyg/men057
Publication status	Published - Nov 2008

Keywords

interlaboratory method comparison
isocyanates
liquid chromatography mass spectrometry
DBA

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10.1093/annhyg/men057

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Cite this

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title = "Validation of transferability of DBA derivatization and LC-MS/MS determination method for isocyanates via an interlaboratory comparison.",

abstract = "An adapted method for the quantitative determination of isocyanates in air was implemented and validated in-house. The method was based on air sampling using an impinger flask containing di-n-butylamine (DBA) in toluene and a glass fibre filter in series. The DBA derivatives were determined using liquid chromatography and tandem mass spectrometry. Studied isocyanates were isophorone diisocyanate, isocyanic acid (ICA), methyl isocyanate, ethyl isocyanate, propyl isocyanate, hexamethylene diisocyanate (HDI), 2,6- and 2,4-toluene diisocyanate, 4,4'-methylene diphenyl diisocyanate (MDI), phenyl isocyanate (PhI), MDI oligomers and different HDI adducts. Monitoring of selected reactions resulted in quantifications with correlation coefficients >0.995, within-batch relative standard deviation (RSD) of repeatability was 500 samples.",

keywords = "interlaboratory method comparison, isocyanates, liquid chromatography mass spectrometry, DBA",

author = "I. Bobeldijk and D. Karlsson and A. Pronk and J. Gonsalves and M. Hekman and {van de Lagemaat}, D. and L. Preller and D. Heederik and G. Skarping",

year = "2008",

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doi = "10.1093/annhyg/men057",

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T1 - Validation of transferability of DBA derivatization and LC-MS/MS determination method for isocyanates via an interlaboratory comparison.

AU - Bobeldijk, I.

AU - Karlsson, D.

AU - Pronk, A.

AU - Gonsalves, J.

AU - Hekman, M.

AU - van de Lagemaat, D.

AU - Preller, L.

AU - Heederik, D.

AU - Skarping, G.

PY - 2008/11

Y1 - 2008/11

N2 - An adapted method for the quantitative determination of isocyanates in air was implemented and validated in-house. The method was based on air sampling using an impinger flask containing di-n-butylamine (DBA) in toluene and a glass fibre filter in series. The DBA derivatives were determined using liquid chromatography and tandem mass spectrometry. Studied isocyanates were isophorone diisocyanate, isocyanic acid (ICA), methyl isocyanate, ethyl isocyanate, propyl isocyanate, hexamethylene diisocyanate (HDI), 2,6- and 2,4-toluene diisocyanate, 4,4'-methylene diphenyl diisocyanate (MDI), phenyl isocyanate (PhI), MDI oligomers and different HDI adducts. Monitoring of selected reactions resulted in quantifications with correlation coefficients >0.995, within-batch relative standard deviation (RSD) of repeatability was 500 samples.

AB - An adapted method for the quantitative determination of isocyanates in air was implemented and validated in-house. The method was based on air sampling using an impinger flask containing di-n-butylamine (DBA) in toluene and a glass fibre filter in series. The DBA derivatives were determined using liquid chromatography and tandem mass spectrometry. Studied isocyanates were isophorone diisocyanate, isocyanic acid (ICA), methyl isocyanate, ethyl isocyanate, propyl isocyanate, hexamethylene diisocyanate (HDI), 2,6- and 2,4-toluene diisocyanate, 4,4'-methylene diphenyl diisocyanate (MDI), phenyl isocyanate (PhI), MDI oligomers and different HDI adducts. Monitoring of selected reactions resulted in quantifications with correlation coefficients >0.995, within-batch relative standard deviation (RSD) of repeatability was 500 samples.

KW - interlaboratory method comparison

KW - isocyanates

KW - liquid chromatography mass spectrometry

KW - DBA

U2 - 10.1093/annhyg/men057

DO - 10.1093/annhyg/men057

M3 - Article

SN - 0003-4878

VL - 52

SP - 757

EP - 763

JO - Annals of Occupational Hygiene

JF - Annals of Occupational Hygiene

IS - 8

ER -