Use of influenza A viruses expressing reporter genes to assess the frequency of double infections in vitro

R Bodewes; N J Nieuwkoop; R J Verburgh; R A M Fouchier; A D M E Osterhaus; G F Rimmelzwaan

doi:10.1099/vir.0.042671-0

Use of influenza A viruses expressing reporter genes to assess the frequency of double infections in vitro

R Bodewes
, N J Nieuwkoop
, R J Verburgh
, R A M Fouchier
, A D M E Osterhaus
, G F Rimmelzwaan

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Exchange of gene segments between mammalian and avian influenza A viruses may lead to the emergence of potential pandemic influenza viruses. Since co-infection of single cells with two viruses is a prerequisite for reassortment to take place, we assessed frequencies of double-infection in vitro using influenza A/H5N1 and A/H1N1 viruses expressing the reporter genes eGFP or mCherry. Double-infected A549 and Madin-Darby canine kidney cells were detected by confocal microscopy and flow cytometry.

Original language	English
Pages (from-to)	1645-8
Number of pages	4
Journal	Journal of General Virology
Volume	93
Issue number	Pt 8
DOIs	https://doi.org/10.1099/vir.0.042671-0
Publication status	Published - Aug 2012

Keywords

Animals
Cell Line
Cell Line, Tumor
Dogs
Flow Cytometry
Gene Expression Regulation, Viral
Genes, Reporter
Genes, Viral
Green Fluorescent Proteins
Humans
Influenza A Virus, H1N1 Subtype
Influenza A Virus, H5N1 Subtype
Luminescent Proteins
Microscopy, Confocal
Reassortant Viruses

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10.1099/vir.0.042671-0

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title = "Use of influenza A viruses expressing reporter genes to assess the frequency of double infections in vitro",

abstract = "Exchange of gene segments between mammalian and avian influenza A viruses may lead to the emergence of potential pandemic influenza viruses. Since co-infection of single cells with two viruses is a prerequisite for reassortment to take place, we assessed frequencies of double-infection in vitro using influenza A/H5N1 and A/H1N1 viruses expressing the reporter genes eGFP or mCherry. Double-infected A549 and Madin-Darby canine kidney cells were detected by confocal microscopy and flow cytometry.",

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year = "2012",

month = aug,

doi = "10.1099/vir.0.042671-0",

language = "English",

volume = "93",

pages = "1645--8",

journal = "Journal of General Virology",

issn = "0022-1317",

publisher = "Microbiology Society",

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T1 - Use of influenza A viruses expressing reporter genes to assess the frequency of double infections in vitro

AU - Bodewes, R

AU - Nieuwkoop, N J

AU - Verburgh, R J

AU - Fouchier, R A M

AU - Osterhaus, A D M E

AU - Rimmelzwaan, G F

PY - 2012/8

Y1 - 2012/8

N2 - Exchange of gene segments between mammalian and avian influenza A viruses may lead to the emergence of potential pandemic influenza viruses. Since co-infection of single cells with two viruses is a prerequisite for reassortment to take place, we assessed frequencies of double-infection in vitro using influenza A/H5N1 and A/H1N1 viruses expressing the reporter genes eGFP or mCherry. Double-infected A549 and Madin-Darby canine kidney cells were detected by confocal microscopy and flow cytometry.

AB - Exchange of gene segments between mammalian and avian influenza A viruses may lead to the emergence of potential pandemic influenza viruses. Since co-infection of single cells with two viruses is a prerequisite for reassortment to take place, we assessed frequencies of double-infection in vitro using influenza A/H5N1 and A/H1N1 viruses expressing the reporter genes eGFP or mCherry. Double-infected A549 and Madin-Darby canine kidney cells were detected by confocal microscopy and flow cytometry.

KW - Animals

KW - Cell Line

KW - Cell Line, Tumor

KW - Dogs

KW - Flow Cytometry

KW - Gene Expression Regulation, Viral

KW - Genes, Reporter

KW - Genes, Viral

KW - Green Fluorescent Proteins

KW - Humans

KW - Influenza A Virus, H1N1 Subtype

KW - Influenza A Virus, H5N1 Subtype

KW - Luminescent Proteins

KW - Microscopy, Confocal

KW - Reassortant Viruses

U2 - 10.1099/vir.0.042671-0

DO - 10.1099/vir.0.042671-0

M3 - Article

C2 - 22535774

SN - 0022-1317

VL - 93

SP - 1645

EP - 1648

JO - Journal of General Virology

JF - Journal of General Virology

IS - Pt 8

ER -

Use of influenza A viruses expressing reporter genes to assess the frequency of double infections in vitro

Abstract

Keywords

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