Abstract
Exchange of gene segments between mammalian and avian influenza A viruses may lead to the emergence of potential pandemic influenza viruses. Since co-infection of single cells with two viruses is a prerequisite for reassortment to take place, we assessed frequencies of double-infection in vitro using influenza A/H5N1 and A/H1N1 viruses expressing the reporter genes eGFP or mCherry. Double-infected A549 and Madin-Darby canine kidney cells were detected by confocal microscopy and flow cytometry.
Original language | English |
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Pages (from-to) | 1645-8 |
Number of pages | 4 |
Journal | Journal of General Virology |
Volume | 93 |
Issue number | Pt 8 |
DOIs | |
Publication status | Published - Aug 2012 |
Keywords
- Animals
- Cell Line
- Cell Line, Tumor
- Dogs
- Flow Cytometry
- Gene Expression Regulation, Viral
- Genes, Reporter
- Genes, Viral
- Green Fluorescent Proteins
- Humans
- Influenza A Virus, H1N1 Subtype
- Influenza A Virus, H5N1 Subtype
- Luminescent Proteins
- Microscopy, Confocal
- Reassortant Viruses