Use of influenza A viruses expressing reporter genes to assess the frequency of double infections in vitro

R Bodewes, N J Nieuwkoop, R J Verburgh, R A M Fouchier, A D M E Osterhaus, G F Rimmelzwaan

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    Exchange of gene segments between mammalian and avian influenza A viruses may lead to the emergence of potential pandemic influenza viruses. Since co-infection of single cells with two viruses is a prerequisite for reassortment to take place, we assessed frequencies of double-infection in vitro using influenza A/H5N1 and A/H1N1 viruses expressing the reporter genes eGFP or mCherry. Double-infected A549 and Madin-Darby canine kidney cells were detected by confocal microscopy and flow cytometry.

    Original languageEnglish
    Pages (from-to)1645-8
    Number of pages4
    JournalJournal of General Virology
    Volume93
    Issue numberPt 8
    DOIs
    Publication statusPublished - Aug 2012

    Keywords

    • Animals
    • Cell Line
    • Cell Line, Tumor
    • Dogs
    • Flow Cytometry
    • Gene Expression Regulation, Viral
    • Genes, Reporter
    • Genes, Viral
    • Green Fluorescent Proteins
    • Humans
    • Influenza A Virus, H1N1 Subtype
    • Influenza A Virus, H5N1 Subtype
    • Luminescent Proteins
    • Microscopy, Confocal
    • Reassortant Viruses

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