Unexpected role of glycosphingo-lipids in cell physiology

G. van Meer, J. Wolthoorn, S. Groux-Degroote, D. van den Heuvel, H.C. Gerritsen, D. Halter, S. Neumann, S. van der Poel, H. Sprong

Research output: Contribution to conferenceAbstractOther research output


We found that natural glucosylceramide does not cross the Golgi membrane and is not a substrate for the multidrug transporters ABCB1 and C1. Instead, glucosylceramide was transported back to the ER by a mechanism involving the glucosylceramide binding protein FAPP2 bound to the trans- Golgi via PI4P. It then crosses towards the ER lumen and reaches the Golgi lumen by vesicular transport, possibly generating ER sphingolipid domains along the way. Meanwhile, glucosylceramide is required for protein sorting in the secretory pathway by affecting conditions in the lumen. As a possible explanation, we found that glucosylceramide affects the lumenal pH.
Original languageUndefined/Unknown
Number of pages1
Publication statusPublished - 2009
EventUnknown event - Asilomar, USA
Duration: 1 Jan 2009 → …


ConferenceUnknown event
CityAsilomar, USA
Period1/01/09 → …

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