Ultralong transients enhance sensitivity and resolution in Orbitrap-based single-ion mass spectrometry

Evolène Deslignière; Victor C Yin; Eduard H T M Ebberink; Amber D Rolland; Arjan Barendregt; Tobias P Wörner; Konstantin O Nagornov; Anton N Kozhinov; Kyle L Fort; Yury O Tsybin; Alexander A Makarov; Albert J R Heck

doi:10.1038/s41592-024-02207-8

Ultralong transients enhance sensitivity and resolution in Orbitrap-based single-ion mass spectrometry

Evolène Deslignière, Victor C Yin^*, Eduard H T M Ebberink, Amber D Rolland, Arjan Barendregt, Tobias P Wörner, Konstantin O Nagornov, Anton N Kozhinov, Kyle L Fort, Yury O Tsybin, Alexander A Makarov, Albert J R Heck^*

^*Corresponding author for this work

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Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Orbitrap-based charge detection mass spectrometry utilizes single-molecule sensitivity to enable mass analysis of even highly heterogeneous, high-mass macromolecular assemblies. For contemporary Orbitrap instruments, the accessible ion detection (recording) times are maximally ~1-2 s. Here by modifying a data acquisition method on an Orbitrap ultrahigh mass range mass spectrometer, we trapped and monitored individual (single) ions for up to 25 s, resulting in a corresponding and huge improvement in signal-to-noise ratio (×5 compared with 1 s), mass resolution (×25) and accuracy in charge and mass determination of Orbitrap-based charge detection mass spectrometry.

Original language	English
Pages (from-to)	619-622
Number of pages	4
Journal	Nature Methods
Volume	21
Issue number	4
Early online date	5 Mar 2024
DOIs	https://doi.org/10.1038/s41592-024-02207-8
Publication status	Published - 2024

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s41592-024-02207-8Final published version, 2.5 MBLicence: Taverne

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Deslignière, E., Yin, V. C., Ebberink, E. H. T. M., Rolland, A. D., Barendregt, A., Wörner, T. P., Nagornov, K. O., Kozhinov, A. N., Fort, K. L., Tsybin, Y. O., Makarov, A. A., & Heck, A. J. R. (2024). Ultralong transients enhance sensitivity and resolution in Orbitrap-based single-ion mass spectrometry. Nature Methods, 21(4), 619-622. https://doi.org/10.1038/s41592-024-02207-8

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abstract = "Orbitrap-based charge detection mass spectrometry utilizes single-molecule sensitivity to enable mass analysis of even highly heterogeneous, high-mass macromolecular assemblies. For contemporary Orbitrap instruments, the accessible ion detection (recording) times are maximally ~1-2 s. Here by modifying a data acquisition method on an Orbitrap ultrahigh mass range mass spectrometer, we trapped and monitored individual (single) ions for up to 25 s, resulting in a corresponding and huge improvement in signal-to-noise ratio (×5 compared with 1 s), mass resolution (×25) and accuracy in charge and mass determination of Orbitrap-based charge detection mass spectrometry.",

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AU - Deslignière, Evolène

AU - Yin, Victor C

AU - Ebberink, Eduard H T M

AU - Rolland, Amber D

AU - Barendregt, Arjan

AU - Wörner, Tobias P

AU - Nagornov, Konstantin O

AU - Kozhinov, Anton N

AU - Fort, Kyle L

AU - Tsybin, Yury O

AU - Makarov, Alexander A

AU - Heck, Albert J R

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Ultralong transients enhance sensitivity and resolution in Orbitrap-based single-ion mass spectrometry

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