Two dominant inhibitory mutants of p21ras interfere with insulin-induced gene expression

R H Medema, R Wubbolts, J L Bos

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    Insulin induces a rapid activation of p21ras in NIH 3T3 and Chinese hamster ovary cells that overexpress the insulin receptor. Previously, we suggested that p21ras may mediate insulin-induced gene expression. To test such a function of p21ras more directly, we studied the effect of different dominant inhibitory mutants of p21ras on the induction of gene expression in response to insulin. We transfected a collagenase promoter-chloramphenicol acetyltransferase (CAT) gene or a fos promoter-luciferase gene into NIH 3T3 cells that overexpressed the insulin receptor. The activities of both promoters were strongly induced after treatment with insulin. This induction could be suppressed by cotransfection of two inhibitory mutant ras genes, H-ras(Asn-17) or H-ras(Leu-61,Ser-186). In particular, insulin-induced activation of the fos promoter was inhibited completely by H-ras(Asn-17). These results show that p21ras functions as an intermediate in the insulin signal transduction route leading to the induction of gene expression.

    Original languageEnglish
    Pages (from-to)5963-7
    Number of pages5
    JournalMolecular and Cellular Biology
    Volume11
    Issue number12
    Publication statusPublished - Dec 1991

    Keywords

    • 3T3 Cells
    • Animals
    • Cell Line
    • Chloramphenicol O-Acetyltransferase
    • Cloning, Molecular
    • Gene Expression Regulation
    • Genes, Dominant
    • HeLa Cells
    • Humans
    • Insulin
    • Mice
    • Microbial Collagenase
    • Mutation
    • Promoter Regions, Genetic
    • Proto-Oncogene Proteins p21(ras)
    • Receptor, Insulin
    • Signal Transduction
    • Transfection

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