TRANSMISSION OF HEPATITIS B FROM HEPATITIS-B-SERONEGATIVE SUBJECTS

V. Thiers; D. Kremsdorf; H. Schellekens; A. Goudeau; J. Sninsky; E. Nakajima; D. Mack; F. Driss; J. Wands; P. Tiollais; C. Brechot

doi:10.1016/S0140-6736(88)92891-7

TRANSMISSION OF HEPATITIS B FROM HEPATITIS-B-SERONEGATIVE SUBJECTS

V. Thiers^*
, D. Kremsdorf
, H. Schellekens
, A. Goudeau
, J. Sninsky
, E. Nakajima
, D. Mack
, F. Driss
, J. Wands
, P. Tiollais
, C. Brechot

^*Corresponding author for this work

Institut Pasteur Paris
Université Paris 5
extern
Virology Laboratory (CNRSUA271)
Novartis
Hopital Laennec
Massachussets General Hospital

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The polymerase chain reaction (PCR) was used to identify and characterise serum HBV DNA sequences in 3 patients negative for all HBV serological markers. HBsAg determinants were detected in 1 individual by monoclonal anti-HBsAg immuno-radiometric assay. By use of sets of primers on the S and pre-S parts of the HBV genome the presence of HBV DNA was demonstrated in the serum of all 3 patients. Inoculation of human sera to 2 chimpanzees induced acute hepatitis in both animals; 1 became positive for HBsAg and anti-HBcAg and the other only for anti-HBsAg. Cloning of DNA sequences from viral isolates from 1 chimpanzee and 1 patient was accomplished after amplification of the 3' region of the S gene. Comparison of the partial nucleotide sequence with that of known HBV subtypes showed 0 and 1 point mutation, respectively, in the highly conserved 3' end of the S gene. Therefore the results show that PCR with HBV primers may unambigously identify HBV infectious particles among non-A, non-B viruses and is a potentially useful diagnostic test for detection of HBV DNA sequences in serum.

Original language	English
Pages (from-to)	1273-1276
Number of pages	4
Journal	The Lancet
Volume	332
Issue number	8623
DOIs	https://doi.org/10.1016/S0140-6736(88)92891-7
Publication status	Published - 3 Dec 1988
Externally published	Yes

Funding

group. It also receives support from the Department of Health and the Chest, Heart and Stroke Association. The alcohol-related studies are supported by the Institute of Alcohol Studies. We are grateful to Prof. Stuart Pocock and Dr Andrew Phillips for their advice in preparing the final version of this paper.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1016/S0140-6736(88)92891-7

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title = "TRANSMISSION OF HEPATITIS B FROM HEPATITIS-B-SERONEGATIVE SUBJECTS",

abstract = "The polymerase chain reaction (PCR) was used to identify and characterise serum HBV DNA sequences in 3 patients negative for all HBV serological markers. HBsAg determinants were detected in 1 individual by monoclonal anti-HBsAg immuno-radiometric assay. By use of sets of primers on the S and pre-S parts of the HBV genome the presence of HBV DNA was demonstrated in the serum of all 3 patients. Inoculation of human sera to 2 chimpanzees induced acute hepatitis in both animals; 1 became positive for HBsAg and anti-HBcAg and the other only for anti-HBsAg. Cloning of DNA sequences from viral isolates from 1 chimpanzee and 1 patient was accomplished after amplification of the 3' region of the S gene. Comparison of the partial nucleotide sequence with that of known HBV subtypes showed 0 and 1 point mutation, respectively, in the highly conserved 3' end of the S gene. Therefore the results show that PCR with HBV primers may unambigously identify HBV infectious particles among non-A, non-B viruses and is a potentially useful diagnostic test for detection of HBV DNA sequences in serum.",

author = "V. Thiers and D. Kremsdorf and H. Schellekens and A. Goudeau and J. Sninsky and E. Nakajima and D. Mack and F. Driss and J. Wands and P. Tiollais and C. Brechot",

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doi = "10.1016/S0140-6736(88)92891-7",

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AU - Thiers, V.

AU - Kremsdorf, D.

AU - Schellekens, H.

AU - Goudeau, A.

AU - Sninsky, J.

AU - Nakajima, E.

AU - Mack, D.

AU - Driss, F.

AU - Wands, J.

AU - Tiollais, P.

AU - Brechot, C.

PY - 1988/12/3

Y1 - 1988/12/3

N2 - The polymerase chain reaction (PCR) was used to identify and characterise serum HBV DNA sequences in 3 patients negative for all HBV serological markers. HBsAg determinants were detected in 1 individual by monoclonal anti-HBsAg immuno-radiometric assay. By use of sets of primers on the S and pre-S parts of the HBV genome the presence of HBV DNA was demonstrated in the serum of all 3 patients. Inoculation of human sera to 2 chimpanzees induced acute hepatitis in both animals; 1 became positive for HBsAg and anti-HBcAg and the other only for anti-HBsAg. Cloning of DNA sequences from viral isolates from 1 chimpanzee and 1 patient was accomplished after amplification of the 3' region of the S gene. Comparison of the partial nucleotide sequence with that of known HBV subtypes showed 0 and 1 point mutation, respectively, in the highly conserved 3' end of the S gene. Therefore the results show that PCR with HBV primers may unambigously identify HBV infectious particles among non-A, non-B viruses and is a potentially useful diagnostic test for detection of HBV DNA sequences in serum.

AB - The polymerase chain reaction (PCR) was used to identify and characterise serum HBV DNA sequences in 3 patients negative for all HBV serological markers. HBsAg determinants were detected in 1 individual by monoclonal anti-HBsAg immuno-radiometric assay. By use of sets of primers on the S and pre-S parts of the HBV genome the presence of HBV DNA was demonstrated in the serum of all 3 patients. Inoculation of human sera to 2 chimpanzees induced acute hepatitis in both animals; 1 became positive for HBsAg and anti-HBcAg and the other only for anti-HBsAg. Cloning of DNA sequences from viral isolates from 1 chimpanzee and 1 patient was accomplished after amplification of the 3' region of the S gene. Comparison of the partial nucleotide sequence with that of known HBV subtypes showed 0 and 1 point mutation, respectively, in the highly conserved 3' end of the S gene. Therefore the results show that PCR with HBV primers may unambigously identify HBV infectious particles among non-A, non-B viruses and is a potentially useful diagnostic test for detection of HBV DNA sequences in serum.

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U2 - 10.1016/S0140-6736(88)92891-7

DO - 10.1016/S0140-6736(88)92891-7

M3 - Article

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SN - 0140-6736

VL - 332

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JO - The Lancet

JF - The Lancet

IS - 8623

ER -

TRANSMISSION OF HEPATITIS B FROM HEPATITIS-B-SERONEGATIVE SUBJECTS

Abstract

Funding

UN SDGs

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