Abstract
Organs-on-chips (OoCs) hold promise to engineer progressively more human-relevant in vitro models for pharmaceutical purposes. Recent developments have delivered increasingly sophisticated designs, yet OoCs still lack in reproducing the inner tissue physiology required to fully resemble the native human body. This review emphasizes the need to include microarchitectural and microstructural features, and discusses promising avenues to incorporate well-defined microarchitectures down to the single-cell level. We highlight how their integration will significantly contribute to the advancement of the field towards highly organized structural and hierarchical tissues-on-chip. We discuss the combination of state-of-the-art micropatterning technologies to achieve OoCs resembling human-intrinsic complexity. It is anticipated that these innovations will yield significant advances in realization of the next generation of OoC models.
Original language | English |
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Pages (from-to) | 739-759 |
Number of pages | 21 |
Journal | Trends in Biotechnology |
Volume | 42 |
Issue number | 6 |
Early online date | 2 Feb 2024 |
DOIs | |
Publication status | Published - Jun 2024 |
Bibliographical note
Publisher Copyright:© 2023 The Author(s)
Funding
The authors acknowledge financial support from the Dutch Research Council via the NWO-TTW Perspective Programme (NWO; Project Number P19-03). R.L. acknowledges funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (Grant Agreement No. 949806, VOLUME‐BIO). The authors declare no conflict of interests. The authors acknowledge financial support from the Dutch Research Council via the NWO-TTW Perspective Programme (NWO; Project Number P19-03). R.L. acknowledges funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (Grant Agreement No. 949806, VOLUME‐BIO).
Funders | Funder number |
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NWO-TTW | P19-03 |
Horizon 2020 Framework Programme | |
European Research Council | |
Nederlandse Organisatie voor Wetenschappelijk Onderzoek | |
Horizon 2020 | 949806 |
Keywords
- biofabrication
- disease models
- laser-induced forward transfer
- microfluidics
- microphysiological systems