TY - JOUR
T1 - Tomato R2R3-MYB proteins SlANT1 and SlAN2
T2 - Same protein activity, different roles
AU - Kiferle, Claudia
AU - Fantini, Elio
AU - Bassolino, Laura
AU - Povero, Giovanni
AU - Spelt, Cornelis
AU - Buti, Sara
AU - Giuliano, Giovanni
AU - Quattrocchio, Francesca
AU - Koes, Ronald
AU - Perata, Pierdomenico
AU - Gonzali, Silvia
PY - 2015/8/26
Y1 - 2015/8/26
N2 - Anthocyanins are water-soluble polyphenolic compounds with a high nutraceutical value. Despite the fact that cultivated tomato varieties do not accumulate anthocyanins in the fruit, the biosynthetic pathway can be activated in the vegetative organs by several environmental stimuli. Little is known about the molecular mechanisms regulating anthocyanin synthesis in tomato. Here, we carried out a molecular and functional characterization of two genes, SlAN2 and SlANT1, encoding two R2R3-MYB transcription factors. We show that both can induce ectopic anthocyanin synthesis in transgenic tomato lines, including the fruit. However, only SlAN2 acts as a positive regulator of anthocyanin synthesis in vegetative tissues under high light or low temperature conditions.
AB - Anthocyanins are water-soluble polyphenolic compounds with a high nutraceutical value. Despite the fact that cultivated tomato varieties do not accumulate anthocyanins in the fruit, the biosynthetic pathway can be activated in the vegetative organs by several environmental stimuli. Little is known about the molecular mechanisms regulating anthocyanin synthesis in tomato. Here, we carried out a molecular and functional characterization of two genes, SlAN2 and SlANT1, encoding two R2R3-MYB transcription factors. We show that both can induce ectopic anthocyanin synthesis in transgenic tomato lines, including the fruit. However, only SlAN2 acts as a positive regulator of anthocyanin synthesis in vegetative tissues under high light or low temperature conditions.
KW - tomato
KW - anthocyanins
KW - R2R3-MYB
UR - http://www.scopus.com/inward/record.url?scp=84943390602&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0136365
DO - 10.1371/journal.pone.0136365
M3 - Article
AN - SCOPUS:84943390602
SN - 1932-6203
VL - 10
JO - PLoS One
JF - PLoS One
IS - 8
M1 - e0136365
ER -