TOM software toolbox: acquisition and analysis for electron tomography

Stephan Nickell; Friedrich Förster; Alexandros Linaroudis; William Del Net; Florian Beck; Reiner Hegerl; Wolfgang Baumeister; Jürgen M Plitzko

doi:10.1016/j.jsb.2004.10.006

TOM software toolbox: acquisition and analysis for electron tomography

Stephan Nickell, Friedrich Förster, Alexandros Linaroudis, William Del Net, Florian Beck, Reiner Hegerl, Wolfgang Baumeister, Jürgen M Plitzko

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Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Automated data acquisition procedures have changed the perspectives of electron tomography (ET) in a profound manner. Elaborate data acquisition schemes with autotuning functions minimize exposure of the specimen to the electron beam and sophisticated image analysis routines retrieve a maximum of information from noisy data sets. "TOM software toolbox" integrates established algorithms and new concepts tailored to the special needs of low dose ET. It provides a user-friendly unified platform for all processing steps: acquisition, alignment, reconstruction, and analysis. Designed as a collection of computational procedures it is a complete software solution within a highly flexible framework. TOM represents a new way of working with the electron microscope and can serve as the basis for future high-throughput applications.

Original language	English
Pages (from-to)	227-34
Number of pages	8
Journal	Journal of Structural Biology
Volume	149
Issue number	3
DOIs	https://doi.org/10.1016/j.jsb.2004.10.006
Publication status	Published - 2005
Externally published	Yes

Keywords

Algorithms
Humans
Image Processing, Computer-Assisted
Software
Tomography
User-Computer Interface

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10.1016/j.jsb.2004.10.006

Cite this

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title = "TOM software toolbox: acquisition and analysis for electron tomography",

abstract = "Automated data acquisition procedures have changed the perspectives of electron tomography (ET) in a profound manner. Elaborate data acquisition schemes with autotuning functions minimize exposure of the specimen to the electron beam and sophisticated image analysis routines retrieve a maximum of information from noisy data sets. {"}TOM software toolbox{"} integrates established algorithms and new concepts tailored to the special needs of low dose ET. It provides a user-friendly unified platform for all processing steps: acquisition, alignment, reconstruction, and analysis. Designed as a collection of computational procedures it is a complete software solution within a highly flexible framework. TOM represents a new way of working with the electron microscope and can serve as the basis for future high-throughput applications.",

keywords = "Algorithms, Humans, Image Processing, Computer-Assisted, Software, Tomography, User-Computer Interface",

author = "Stephan Nickell and Friedrich F{\"o}rster and Alexandros Linaroudis and Net, {William Del} and Florian Beck and Reiner Hegerl and Wolfgang Baumeister and Plitzko, {J{\"u}rgen M}",

year = "2005",

doi = "10.1016/j.jsb.2004.10.006",

language = "English",

volume = "149",

pages = "227--34",

journal = "Journal of Structural Biology",

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publisher = "Academic Press Inc.",

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TY - JOUR

T1 - TOM software toolbox

T2 - acquisition and analysis for electron tomography

AU - Nickell, Stephan

AU - Förster, Friedrich

AU - Linaroudis, Alexandros

AU - Net, William Del

AU - Beck, Florian

AU - Hegerl, Reiner

AU - Baumeister, Wolfgang

AU - Plitzko, Jürgen M

PY - 2005

Y1 - 2005

N2 - Automated data acquisition procedures have changed the perspectives of electron tomography (ET) in a profound manner. Elaborate data acquisition schemes with autotuning functions minimize exposure of the specimen to the electron beam and sophisticated image analysis routines retrieve a maximum of information from noisy data sets. "TOM software toolbox" integrates established algorithms and new concepts tailored to the special needs of low dose ET. It provides a user-friendly unified platform for all processing steps: acquisition, alignment, reconstruction, and analysis. Designed as a collection of computational procedures it is a complete software solution within a highly flexible framework. TOM represents a new way of working with the electron microscope and can serve as the basis for future high-throughput applications.

AB - Automated data acquisition procedures have changed the perspectives of electron tomography (ET) in a profound manner. Elaborate data acquisition schemes with autotuning functions minimize exposure of the specimen to the electron beam and sophisticated image analysis routines retrieve a maximum of information from noisy data sets. "TOM software toolbox" integrates established algorithms and new concepts tailored to the special needs of low dose ET. It provides a user-friendly unified platform for all processing steps: acquisition, alignment, reconstruction, and analysis. Designed as a collection of computational procedures it is a complete software solution within a highly flexible framework. TOM represents a new way of working with the electron microscope and can serve as the basis for future high-throughput applications.

KW - Algorithms

KW - Humans

KW - Image Processing, Computer-Assisted

KW - Software

KW - Tomography

KW - User-Computer Interface

U2 - 10.1016/j.jsb.2004.10.006

DO - 10.1016/j.jsb.2004.10.006

M3 - Article

C2 - 15721576

SN - 1047-8477

VL - 149

SP - 227

EP - 234

JO - Journal of Structural Biology

JF - Journal of Structural Biology

IS - 3

ER -

TOM software toolbox: acquisition and analysis for electron tomography

Abstract

Keywords

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