Tn Antigen Mimics Based on sp2-Iminosugars with Affinity for an anti-MUC1 Antibody

  • Elena M Sánchez Fernández
  • , Claudio D Navo
  • , N. Martinez Saez
  • , Rita Conçalves-Pereira
  • , V.J. Somovilla Busto
  • , Alberto Avenoza
  • , Jesus Arenas Busto
  • , Gonçalo J L Bernardes
  • , Gonzalo Jiménez-Osés
  • , Francisco Corzana
  • , José M. García Fernández
  • , Carmen Ortiz Mellet
  • , Jesús M. Peregrina

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    The first examples of amino acid (Ser/Thr)-sp(2)-iminosugar glycomimetic conjugates featuring an α-O-linked pseudoanomeric linkage are reported. The key synthetic step involves the completely diastereoselective α-glycosylation of Ser/Thr due to strong stereoelectronic and conformational bias imposed by the bicyclic sp(2)-iminosugar scaffold. Mucin-related glycopeptides incorporating these motifs were recognized by the monoclonal antibody (mAb) scFv-SM3, with activities depending on both the hydroxylation pattern (Glc/Gal/GlcNAc/GalNAc) of the sp(2)-iminosugar and the peptide aglycone structure (Ser/Thr).
    Original languageEnglish
    JournalOrganic Letters
    Volume18
    Issue number15
    DOIs
    Publication statusPublished - 5 Aug 2016

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