The transcriptional regulator c2h2 accelerates mushroom formation in Agaricus bisporus

J.F. Pelkmans; A.M. Vos; K Scholtmeijer; Eddy Hendrix; Johan J Baars; Thies Gehrmann; Marcel J Reinders; L.G. Lugones; HAB Wösten

doi:10.1007/s00253-016-7574-9

The transcriptional regulator c2h2 accelerates mushroom formation in Agaricus bisporus

J.F. Pelkmans, A.M. Vos, K Scholtmeijer, Eddy Hendrix, Johan J Baars, Thies Gehrmann, Marcel J Reinders, L.G. Lugones, HAB Wösten

Sub Molecular Microbiology

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.

Original language	English
Pages (from-to)	7151-7159
Journal	Applied Microbiology and Biotechnology
Volume	100
Issue number	16
DOIs	https://doi.org/10.1007/s00253-016-7574-9
Publication status	Published - Aug 2016

Keywords

Fungi
Basidiomycete
Agaricus bisporus
Mushroom
Transcription factor
Cys2His2

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title = "The transcriptional regulator c2h2 accelerates mushroom formation in Agaricus bisporus",

abstract = "The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.",

keywords = "Fungi, Basidiomycete, Agaricus bisporus, Mushroom, Transcription factor, Cys2His2",

author = "J.F. Pelkmans and A.M. Vos and K Scholtmeijer and Eddy Hendrix and Baars, {Johan J} and Thies Gehrmann and Reinders, {Marcel J} and L.G. Lugones and HAB W{\"o}sten",

year = "2016",

month = aug,

doi = "10.1007/s00253-016-7574-9",

language = "English",

volume = "100",

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journal = "Applied Microbiology and Biotechnology",

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T1 - The transcriptional regulator c2h2 accelerates mushroom formation in Agaricus bisporus

AU - Pelkmans, J.F.

AU - Vos, A.M.

AU - Scholtmeijer, K

AU - Hendrix, Eddy

AU - Baars, Johan J

AU - Gehrmann, Thies

AU - Reinders, Marcel J

AU - Lugones, L.G.

AU - Wösten, HAB

PY - 2016/8

Y1 - 2016/8

N2 - The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.

AB - The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.

KW - Fungi

KW - Basidiomycete

KW - Agaricus bisporus

KW - Mushroom

KW - Transcription factor

KW - Cys2His2

U2 - 10.1007/s00253-016-7574-9

DO - 10.1007/s00253-016-7574-9

M3 - Article

SN - 0175-7598

VL - 100

SP - 7151

EP - 7159

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 16

ER -

The transcriptional regulator c2h2 accelerates mushroom formation in Agaricus bisporus

Abstract

Keywords

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