The transcription factor TCFL5 responds to A MYB to elaborate the male meiotic program in mice

Katharine Cecchini; Adriano Biasini; Tianxiong Yu; Martin Säflund; Haiwei Mou; Amena Arif; Atiyeh Eghbali; Cansu Colpan; Ildar Gainetdinov; Dirk G de Rooij; Zhiping Weng; Phillip Zamore; Deniz M Ozata

doi:10.1530/rep-22-0355

The transcription factor TCFL5 responds to A MYB to elaborate the male meiotic program in mice

Katharine Cecchini, Adriano Biasini, Tianxiong Yu, Martin Säflund, Haiwei Mou, Amena Arif, Atiyeh Eghbali, Cansu Colpan, Ildar Gainetdinov, Dirk G de Rooij, Zhiping Weng, Phillip Zamore, Deniz M Ozata

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

In male mice, the transcription factors STRA8 and MEISON initiate meiosis I. We report that STRA8/MEISON activates the transcription factors A-MYB and TCFL5, which together reprogram gene expression after spermatogonia enter into meiosis. TCFL5 promotes the transcription of genes required for meiosis, mRNA turnover, miR-34/449 production, meiotic exit, and spermiogenesis. This transcriptional architecture is conserved in rhesus macaque, suggesting TCFL5 plays a central role in meiosis and spermiogenesis in placental mammals. Tcfl5^em1/em1 mutants are sterile, and spermatogenesis arrests at the mid- or late-pachytene stage of meiosis. Moreover, Tcfl5^+/em1 mutants produce fewer motile sperm.

Original language	English
Article number	REP-22-0355
Pages (from-to)	183-196
Number of pages	14
Journal	Reproduction
Volume	165
Issue number	2
Early online date	1 Nov 2022
DOIs	https://doi.org/10.1530/rep-22-0355
Publication status	Published - 1 Feb 2023

Keywords

TCFL5
spermatogenesis
meiosis
A-MYB
transcription factors network,
miR-34/449
Dna methylation
Motile ciliogenesis
Meiosis
Pirna production
Protein
Dynamics
Network
Clusters
Family
Mili

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10.1530/rep-22-0355

1741-7899-REP-22-0355Final published version, 4.72 MBLicence: Taverne

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title = "The transcription factor TCFL5 responds to A MYB to elaborate the male meiotic program in mice",

abstract = "In male mice, the transcription factors STRA8 and MEISON initiate meiosis I. We report that STRA8/MEISON activates the transcription factors A-MYB and TCFL5, which together reprogram gene expression after spermatogonia enter into meiosis. TCFL5 promotes the transcription of genes required for meiosis, mRNA turnover, miR-34/449 production, meiotic exit, and spermiogenesis. This transcriptional architecture is conserved in rhesus macaque, suggesting TCFL5 plays a central role in meiosis and spermiogenesis in placental mammals. Tcfl5em1/em1 mutants are sterile, and spermatogenesis arrests at the mid- or late-pachytene stage of meiosis. Moreover, Tcfl5+/em1 mutants produce fewer motile sperm.",

keywords = "TCFL5, spermatogenesis, meiosis, A-MYB, transcription factors network,, miR-34/449, Dna methylation, Motile ciliogenesis, Meiosis, Pirna production, Protein, Dynamics, Network, Clusters, Family, Mili",

author = "Katharine Cecchini and Adriano Biasini and Tianxiong Yu and Martin S{\"a}flund and Haiwei Mou and Amena Arif and Atiyeh Eghbali and Cansu Colpan and Ildar Gainetdinov and {de Rooij}, {Dirk G} and Zhiping Weng and Phillip Zamore and Ozata, {Deniz M}",

note = "Funding Information: This work was supported in part by NIGMS grants R37 GM062862 and R35 GM136275 (PDZ), NICHHD grant P01 HD078253 (ZW and PDZ), and Swedish Research Council grant 2020-03818 (DM{\"O}), Carltryggersstiftelse CTS 21:1158 (DM{\"O}). The UMMS FACS Core is supported in part by NIH grant S10OD028576. Publisher Copyright: {\textcopyright} 2023 Society for Reproduction and Fertility.",

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doi = "10.1530/rep-22-0355",

language = "English",

volume = "165",

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journal = "Reproduction",

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T1 - The transcription factor TCFL5 responds to A MYB to elaborate the male meiotic program in mice

AU - Cecchini, Katharine

AU - Biasini, Adriano

AU - Yu, Tianxiong

AU - Säflund, Martin

AU - Mou, Haiwei

AU - Arif, Amena

AU - Eghbali, Atiyeh

AU - Colpan, Cansu

AU - Gainetdinov, Ildar

AU - de Rooij, Dirk G

AU - Weng, Zhiping

AU - Zamore, Phillip

AU - Ozata, Deniz M

N1 - Funding Information: This work was supported in part by NIGMS grants R37 GM062862 and R35 GM136275 (PDZ), NICHHD grant P01 HD078253 (ZW and PDZ), and Swedish Research Council grant 2020-03818 (DMÖ), Carltryggersstiftelse CTS 21:1158 (DMÖ). The UMMS FACS Core is supported in part by NIH grant S10OD028576. Publisher Copyright: © 2023 Society for Reproduction and Fertility.

PY - 2023/2/1

Y1 - 2023/2/1

N2 - In male mice, the transcription factors STRA8 and MEISON initiate meiosis I. We report that STRA8/MEISON activates the transcription factors A-MYB and TCFL5, which together reprogram gene expression after spermatogonia enter into meiosis. TCFL5 promotes the transcription of genes required for meiosis, mRNA turnover, miR-34/449 production, meiotic exit, and spermiogenesis. This transcriptional architecture is conserved in rhesus macaque, suggesting TCFL5 plays a central role in meiosis and spermiogenesis in placental mammals. Tcfl5em1/em1 mutants are sterile, and spermatogenesis arrests at the mid- or late-pachytene stage of meiosis. Moreover, Tcfl5+/em1 mutants produce fewer motile sperm.

AB - In male mice, the transcription factors STRA8 and MEISON initiate meiosis I. We report that STRA8/MEISON activates the transcription factors A-MYB and TCFL5, which together reprogram gene expression after spermatogonia enter into meiosis. TCFL5 promotes the transcription of genes required for meiosis, mRNA turnover, miR-34/449 production, meiotic exit, and spermiogenesis. This transcriptional architecture is conserved in rhesus macaque, suggesting TCFL5 plays a central role in meiosis and spermiogenesis in placental mammals. Tcfl5em1/em1 mutants are sterile, and spermatogenesis arrests at the mid- or late-pachytene stage of meiosis. Moreover, Tcfl5+/em1 mutants produce fewer motile sperm.

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KW - transcription factors network,

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KW - Dna methylation

KW - Motile ciliogenesis

KW - Meiosis

KW - Pirna production

KW - Protein

KW - Dynamics

KW - Network

KW - Clusters

KW - Family

KW - Mili

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JO - Reproduction

JF - Reproduction

IS - 2

M1 - REP-22-0355

ER -

The transcription factor TCFL5 responds to A MYB to elaborate the male meiotic program in mice

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Keywords

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