The styrene-maleic acid copolymer:: a versatile tool in membrane research

Jonas M Dörr; Stefan Scheidelaar; Martijn C Koorengevel; Juan Dominguez Pardo; Marre Schäfer; Cornelis A van Walree; J Antoinette Killian

doi:10.1007/s00249-015-1093-y

The styrene-maleic acid copolymer: a versatile tool in membrane research

Jonas M Dörr, Stefan Scheidelaar, Martijn C Koorengevel, Juan Dominguez Pardo, Marre Schäfer, Cornelis A van Walree, J Antoinette Killian

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A new and promising tool in membrane research is the detergent-free solubilization of membrane proteins by styrene-maleic acid copolymers (SMAs). These amphipathic molecules are able to solubilize lipid bilayers in the form of nanodiscs that are bounded by the polymer. Thus, membrane proteins can be directly extracted from cells in a water-soluble form while conserving a patch of native membrane around them. In this review article, we briefly discuss current methods of membrane protein solubilization and stabilization. We then zoom in on SMAs, describe their physico-chemical properties, and discuss their membrane-solubilizing effect. This is followed by an overview of studies in which SMA has been used to isolate and investigate membrane proteins. Finally, potential future applications of the methodology are discussed for structural and functional studies on membrane proteins in a near-native environment and for characterizing protein-lipid and protein-protein interactions.

Original language	English
Pages (from-to)	3-21
Number of pages	19
Journal	European Biophysics Journal
Volume	45
Issue number	1
Early online date	6 Dec 2015
DOIs	https://doi.org/10.1007/s00249-015-1093-y
Publication status	Published - Jan 2016

Keywords

Membrane proteins
Native nanodiscs
Styrene–maleic acid copolymers
SMALPs Lipid–protein interactions
Lipodisq

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title = "The styrene-maleic acid copolymer:: a versatile tool in membrane research",

abstract = "A new and promising tool in membrane research is the detergent-free solubilization of membrane proteins by styrene-maleic acid copolymers (SMAs). These amphipathic molecules are able to solubilize lipid bilayers in the form of nanodiscs that are bounded by the polymer. Thus, membrane proteins can be directly extracted from cells in a water-soluble form while conserving a patch of native membrane around them. In this review article, we briefly discuss current methods of membrane protein solubilization and stabilization. We then zoom in on SMAs, describe their physico-chemical properties, and discuss their membrane-solubilizing effect. This is followed by an overview of studies in which SMA has been used to isolate and investigate membrane proteins. Finally, potential future applications of the methodology are discussed for structural and functional studies on membrane proteins in a near-native environment and for characterizing protein-lipid and protein-protein interactions.",

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AU - Dörr, Jonas M

AU - Scheidelaar, Stefan

AU - Koorengevel, Martijn C

AU - Dominguez Pardo, Juan

AU - Schäfer, Marre

AU - van Walree, Cornelis A

AU - Killian, J Antoinette

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AB - A new and promising tool in membrane research is the detergent-free solubilization of membrane proteins by styrene-maleic acid copolymers (SMAs). These amphipathic molecules are able to solubilize lipid bilayers in the form of nanodiscs that are bounded by the polymer. Thus, membrane proteins can be directly extracted from cells in a water-soluble form while conserving a patch of native membrane around them. In this review article, we briefly discuss current methods of membrane protein solubilization and stabilization. We then zoom in on SMAs, describe their physico-chemical properties, and discuss their membrane-solubilizing effect. This is followed by an overview of studies in which SMA has been used to isolate and investigate membrane proteins. Finally, potential future applications of the methodology are discussed for structural and functional studies on membrane proteins in a near-native environment and for characterizing protein-lipid and protein-protein interactions.

KW - Membrane proteins

KW - Native nanodiscs

KW - Styrene–maleic acid copolymers

KW - SMALPs Lipid–protein interactions

KW - Lipodisq

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DO - 10.1007/s00249-015-1093-y

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EP - 21

JO - European Biophysics Journal

JF - European Biophysics Journal

IS - 1

ER -

The styrene-maleic acid copolymer: a versatile tool in membrane research

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Keywords

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