TY - JOUR
T1 - The Role of Oviductal Cells in Activating Stallion Spermatozoa
AU - Leemans, Bart
AU - Gadella, Bart M.
AU - Stout, Tom A. E.
AU - De Schauwer, Catharina
AU - Nelis, Hilde
AU - Hoogewijs, Maarten
AU - Van Soom, Ann
PY - 2016/8
Y1 - 2016/8
N2 - Conventional in vitro fertilization is poorly successful with equine gametes. Although stallion spermatozoa bind to the zona pellucida in vitro, they fail to acrosome react and cannot therefore penetrate into the perivitelline space. Failed sperm penetration most likely relates to the absence in in vitro fertilization media of essential molecules required to fully support stallion sperm capacitation. In vivo, the oviductal lumen provides an environment that appropriately regulates interactions between the gametes and promotes fertilization. Identifying the oviductal "fertilization stimulating factors" would enormously benefit the development of equine in vitro fertilization media. This review focuses on the current understanding of equine sperm-oviduct interactions, which may hold essential clues to achieving successful in vitro fertilization with equine gametes. (C) 2016 Elsevier Inc. All rights reserved.
AB - Conventional in vitro fertilization is poorly successful with equine gametes. Although stallion spermatozoa bind to the zona pellucida in vitro, they fail to acrosome react and cannot therefore penetrate into the perivitelline space. Failed sperm penetration most likely relates to the absence in in vitro fertilization media of essential molecules required to fully support stallion sperm capacitation. In vivo, the oviductal lumen provides an environment that appropriately regulates interactions between the gametes and promotes fertilization. Identifying the oviductal "fertilization stimulating factors" would enormously benefit the development of equine in vitro fertilization media. This review focuses on the current understanding of equine sperm-oviduct interactions, which may hold essential clues to achieving successful in vitro fertilization with equine gametes. (C) 2016 Elsevier Inc. All rights reserved.
KW - Equid
KW - Hyperactivated motility
KW - In vitro fertilization
KW - Oviduct
KW - Protein tyrosine phosphorylation
KW - Sperm-oviduct interaction
UR - https://www.scopus.com/pages/publications/84978511877
U2 - 10.1016/j.jevs.2016.06.003
DO - 10.1016/j.jevs.2016.06.003
M3 - Review article
AN - SCOPUS:84978511877
SN - 0737-0806
VL - 43
SP - S49-S55
JO - Journal of Equine Veterinary Science
JF - Journal of Equine Veterinary Science
IS - Supplement
ER -