The Role of B Cells in Carriage and Clearance of Mycoplasma pneumoniae From the Respiratory Tract of Mice

Patrick M Meyer Sauteur; Ruben C A de Groot; Silvia C Estevão; Theo Hoogenboezem; Adrianus C J M de Bruijn; Marcel Sluijter; Marjolein J W de Bruijn; Ismé M De Kleer; Rien van Haperen; Judith M A van den Brand; Debby Bogaert; Pieter L A Fraaij; Cornelis Vink; Rudi W Hendriks; Janneke N Samsom; Wendy W J Unger; Annemarie M C van Rossum

doi:10.1093/infdis/jix559

The Role of B Cells in Carriage and Clearance of Mycoplasma pneumoniae From the Respiratory Tract of Mice

Patrick M Meyer Sauteur, Ruben C A de Groot, Silvia C Estevão, Theo Hoogenboezem, Adrianus C J M de Bruijn, Marcel Sluijter, Marjolein J W de Bruijn, Ismé M De Kleer, Rien van Haperen, Judith M A van den Brand, Debby Bogaert, Pieter L A Fraaij, Cornelis Vink, Rudi W Hendriks, Janneke N Samsom, Wendy W J Unger, Annemarie M C van Rossum

Division of Infectious Diseases and Hospital Epidemiology, Children's Research Center, University Children's Hospital Zurich, Switzerland.
Laboratory of Pediatrics, Erasmus MC University Medical Center-Sophia Children's Hospital, Rotterdam, The Netherlands.
Department of Pulmonary Medicine, University Medical Center, Rotterdam, The Netherlands.
Department of Cell Biology and Genetics, University Medical Center, Rotterdam, The Netherlands.
Erasmus University Medical Center Rotterdam
Department of Pediatric Immunology and Infectious Diseases, Wilhelmina Children Hospital, University Medical Center, Utrecht, The Netherlands.
Erasmus University College
Laboratory of Pediatrics, Division of Gastroenterology and Nutrition, Erasmus MC University Medical Center-Sophia Children's Hospital, Rotterdam, The Netherlands.
Department of Pediatrics, Division of Pediatric Infectious Diseases and Immunology, Erasmus MC University Medical Center-Sophia Children's Hospital, Rotterdam, The Netherlands.

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Background: Carriage of Mycoplasma pneumoniae (Mp) in the nasopharynx is considered a prerequisite for pulmonary infection. It is interesting to note that Mp carriage is also detected after infection. Although B cells are known to be involved in pulmonary Mp clearance, their role in Mp carriage is unknown.

Methods: In this study, we show in a mouse model that Mp persists in the nose after pulmonary infection, similar to humans.

Results: Infection of mice enhanced Mp-specific immunoglobulin (Ig) M and IgG levels in serum and bronchoalveolar lavage fluid. However, nasal washes only contained elevated Mp-specific IgA. These differences in Ig compartmentalization correlated with differences in Mp-specific B cell responses between nose- and lung-draining lymphoid tissues. Moreover, transferred Mp-specific serum Igs had no effect on nasal carriage in B cell-deficient μMT mice, whereas this enabled μMT mice to clear pulmonary Mp infection.

Conclusions: We report the first evidence that humoral immunity is limited in clearing Mp from the upper respiratory tract.

Original language	English
Pages (from-to)	298-309
Number of pages	12
Journal	Journal of Infectious Diseases
Volume	217
Issue number	2
DOIs	https://doi.org/10.1093/infdis/jix559
Publication status	Published - 15 Jan 2018
Externally published	Yes

Keywords

B cells
carriage
Mycoplasma pneumoniae
mouse model
pneumonia

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10.1093/infdis/jix559

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Meyer Sauteur, P. M., de Groot, R. C. A., Estevão, S. C., Hoogenboezem, T., de Bruijn, A. C. J. M., Sluijter, M., de Bruijn, M. J. W., De Kleer, I. M., van Haperen, R., van den Brand, J. M. A., Bogaert, D., Fraaij, P. L. A., Vink, C., Hendriks, R. W., Samsom, J. N., Unger, W. W. J., & van Rossum, A. M. C. (2018). The Role of B Cells in Carriage and Clearance of Mycoplasma pneumoniae From the Respiratory Tract of Mice. Journal of Infectious Diseases, 217(2), 298-309. https://doi.org/10.1093/infdis/jix559

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title = "The Role of B Cells in Carriage and Clearance of Mycoplasma pneumoniae From the Respiratory Tract of Mice",

abstract = "Background: Carriage of Mycoplasma pneumoniae (Mp) in the nasopharynx is considered a prerequisite for pulmonary infection. It is interesting to note that Mp carriage is also detected after infection. Although B cells are known to be involved in pulmonary Mp clearance, their role in Mp carriage is unknown.Methods: In this study, we show in a mouse model that Mp persists in the nose after pulmonary infection, similar to humans.Results: Infection of mice enhanced Mp-specific immunoglobulin (Ig) M and IgG levels in serum and bronchoalveolar lavage fluid. However, nasal washes only contained elevated Mp-specific IgA. These differences in Ig compartmentalization correlated with differences in Mp-specific B cell responses between nose- and lung-draining lymphoid tissues. Moreover, transferred Mp-specific serum Igs had no effect on nasal carriage in B cell-deficient μMT mice, whereas this enabled μMT mice to clear pulmonary Mp infection.Conclusions: We report the first evidence that humoral immunity is limited in clearing Mp from the upper respiratory tract.",

keywords = "B cells, carriage, Mycoplasma pneumoniae, mouse model, pneumonia",

author = "{Meyer Sauteur}, {Patrick M} and {de Groot}, {Ruben C A} and Estev{\~a}o, {Silvia C} and Theo Hoogenboezem and {de Bruijn}, {Adrianus C J M} and Marcel Sluijter and {de Bruijn}, {Marjolein J W} and {De Kleer}, {Ism{\'e} M} and {van Haperen}, Rien and {van den Brand}, {Judith M A} and Debby Bogaert and Fraaij, {Pieter L A} and Cornelis Vink and Hendriks, {Rudi W} and Samsom, {Janneke N} and Unger, {Wendy W J} and {van Rossum}, {Annemarie M C}",

year = "2018",

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day = "15",

doi = "10.1093/infdis/jix559",

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Meyer Sauteur, PM, de Groot, RCA, Estevão, SC, Hoogenboezem, T, de Bruijn, ACJM, Sluijter, M, de Bruijn, MJW, De Kleer, IM, van Haperen, R, van den Brand, JMA, Bogaert, D, Fraaij, PLA, Vink, C, Hendriks, RW, Samsom, JN, Unger, WWJ & van Rossum, AMC 2018, 'The Role of B Cells in Carriage and Clearance of Mycoplasma pneumoniae From the Respiratory Tract of Mice', Journal of Infectious Diseases, vol. 217, no. 2, pp. 298-309. https://doi.org/10.1093/infdis/jix559

TY - JOUR

T1 - The Role of B Cells in Carriage and Clearance of Mycoplasma pneumoniae From the Respiratory Tract of Mice

AU - Meyer Sauteur, Patrick M

AU - de Groot, Ruben C A

AU - Estevão, Silvia C

AU - Hoogenboezem, Theo

AU - de Bruijn, Adrianus C J M

AU - Sluijter, Marcel

AU - de Bruijn, Marjolein J W

AU - De Kleer, Ismé M

AU - van Haperen, Rien

AU - van den Brand, Judith M A

AU - Bogaert, Debby

AU - Fraaij, Pieter L A

AU - Vink, Cornelis

AU - Hendriks, Rudi W

AU - Samsom, Janneke N

AU - Unger, Wendy W J

AU - van Rossum, Annemarie M C

PY - 2018/1/15

Y1 - 2018/1/15

N2 - Background: Carriage of Mycoplasma pneumoniae (Mp) in the nasopharynx is considered a prerequisite for pulmonary infection. It is interesting to note that Mp carriage is also detected after infection. Although B cells are known to be involved in pulmonary Mp clearance, their role in Mp carriage is unknown.Methods: In this study, we show in a mouse model that Mp persists in the nose after pulmonary infection, similar to humans.Results: Infection of mice enhanced Mp-specific immunoglobulin (Ig) M and IgG levels in serum and bronchoalveolar lavage fluid. However, nasal washes only contained elevated Mp-specific IgA. These differences in Ig compartmentalization correlated with differences in Mp-specific B cell responses between nose- and lung-draining lymphoid tissues. Moreover, transferred Mp-specific serum Igs had no effect on nasal carriage in B cell-deficient μMT mice, whereas this enabled μMT mice to clear pulmonary Mp infection.Conclusions: We report the first evidence that humoral immunity is limited in clearing Mp from the upper respiratory tract.

AB - Background: Carriage of Mycoplasma pneumoniae (Mp) in the nasopharynx is considered a prerequisite for pulmonary infection. It is interesting to note that Mp carriage is also detected after infection. Although B cells are known to be involved in pulmonary Mp clearance, their role in Mp carriage is unknown.Methods: In this study, we show in a mouse model that Mp persists in the nose after pulmonary infection, similar to humans.Results: Infection of mice enhanced Mp-specific immunoglobulin (Ig) M and IgG levels in serum and bronchoalveolar lavage fluid. However, nasal washes only contained elevated Mp-specific IgA. These differences in Ig compartmentalization correlated with differences in Mp-specific B cell responses between nose- and lung-draining lymphoid tissues. Moreover, transferred Mp-specific serum Igs had no effect on nasal carriage in B cell-deficient μMT mice, whereas this enabled μMT mice to clear pulmonary Mp infection.Conclusions: We report the first evidence that humoral immunity is limited in clearing Mp from the upper respiratory tract.

KW - B cells

KW - carriage

KW - Mycoplasma pneumoniae

KW - mouse model

KW - pneumonia

U2 - 10.1093/infdis/jix559

DO - 10.1093/infdis/jix559

M3 - Article

C2 - 29099932

SN - 0022-1899

VL - 217

SP - 298

EP - 309

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 2

ER -

The Role of B Cells in Carriage and Clearance of Mycoplasma pneumoniae From the Respiratory Tract of Mice

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Keywords

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