The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors

Xiaoyi Chen; Juan P. Moran Torres; Peter Jan Vonk; J. Mirjam A. Damen; Karli R. Reiding; Jan Dijksterhuis; Luis G. Lugones; Han A.B. Wösten

doi:10.1016/j.fgb.2024.103894

The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors

Xiaoyi Chen, Juan P. Moran Torres, Peter Jan Vonk, J. Mirjam A. Damen, Karli R. Reiding, Jan Dijksterhuis, Luis G. Lugones, Han A.B. Wösten^*

^*Corresponding author for this work

Centraalbureau voor Schimmelcultures

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Inactivation of flbA in Aspergillus niger results in thinner cell walls, increased cell lysis, abolished sporulation, and an increased secretome complexity. A total of 36 transcription factor (TF) genes are differentially expressed in ΔflbA. Here, seven of these genes (abaA, aslA, aslB, azf1, htfA, nosA, and srbA) were inactivated. Inactivation of each of these genes affected sporulation and, with the exception of abaA, cell wall integrity and protein secretion. The impact on secretion was strongest in the case of ΔaslA and ΔaslB that showed increased pepsin, cellulase, and amylase activity. Biomass was reduced of agar cultures of ΔabaA, ΔaslA, ΔnosA, and ΔsrbA, while biomass was higher in liquid shaken cultures of ΔaslA and ΔaslB. The ΔaslA and ΔhtfA strains showed increased resistance to H₂O₂, while ΔaslB was more sensitive to this reactive oxygen species. Together, inactivation of the seven TF genes impacted biomass formation, sporulation, protein secretion, and stress resistance, and thereby these genes explain at least part of the pleiotropic phenotype of ΔflbA of A. niger.

Original language	English
Article number	103894
Number of pages	13
Journal	Fungal Genetics and Biology
Volume	172
DOIs	https://doi.org/10.1016/j.fgb.2024.103894
Publication status	Published - Jun 2024

Bibliographical note

Publisher Copyright:
© 2024 The Author(s)

Funding

This work was supported by the China Scholarship Council CSC to Xiaoyi Chen and by Consejo Nacional de Ciencia y Tecnolog\u00EDa CONACYT to Juan P. Moran Torres.

Funders	Funder number
China Scholarship Council
Consejo Nacional de Humanidades, Ciencias y Tecnologías

Keywords

Asexual development
Aspergillus
Cell wall stress
FlbA
Fungus
Secretion

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1-s2.0-S1087184524000318-mainFinal published version, 8.04 MBLicence: CC BY

Cite this

Chen, X., Moran Torres, J. P., Jan Vonk, P., Damen, J. M. A., Reiding, K. R., Dijksterhuis, J., Lugones, L. G., & Wösten, H. A. B. (2024). The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors. Fungal Genetics and Biology, 172, Article 103894. https://doi.org/10.1016/j.fgb.2024.103894

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abstract = "Inactivation of flbA in Aspergillus niger results in thinner cell walls, increased cell lysis, abolished sporulation, and an increased secretome complexity. A total of 36 transcription factor (TF) genes are differentially expressed in ΔflbA. Here, seven of these genes (abaA, aslA, aslB, azf1, htfA, nosA, and srbA) were inactivated. Inactivation of each of these genes affected sporulation and, with the exception of abaA, cell wall integrity and protein secretion. The impact on secretion was strongest in the case of ΔaslA and ΔaslB that showed increased pepsin, cellulase, and amylase activity. Biomass was reduced of agar cultures of ΔabaA, ΔaslA, ΔnosA, and ΔsrbA, while biomass was higher in liquid shaken cultures of ΔaslA and ΔaslB. The ΔaslA and ΔhtfA strains showed increased resistance to H2O2, while ΔaslB was more sensitive to this reactive oxygen species. Together, inactivation of the seven TF genes impacted biomass formation, sporulation, protein secretion, and stress resistance, and thereby these genes explain at least part of the pleiotropic phenotype of ΔflbA of A. niger.",

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author = "Xiaoyi Chen and {Moran Torres}, {Juan P.} and {Jan Vonk}, Peter and Damen, {J. Mirjam A.} and Reiding, {Karli R.} and Jan Dijksterhuis and Lugones, {Luis G.} and W{\"o}sten, {Han A.B.}",

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AU - Chen, Xiaoyi

AU - Moran Torres, Juan P.

AU - Jan Vonk, Peter

AU - Damen, J. Mirjam A.

AU - Reiding, Karli R.

AU - Dijksterhuis, Jan

AU - Lugones, Luis G.

AU - Wösten, Han A.B.

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N2 - Inactivation of flbA in Aspergillus niger results in thinner cell walls, increased cell lysis, abolished sporulation, and an increased secretome complexity. A total of 36 transcription factor (TF) genes are differentially expressed in ΔflbA. Here, seven of these genes (abaA, aslA, aslB, azf1, htfA, nosA, and srbA) were inactivated. Inactivation of each of these genes affected sporulation and, with the exception of abaA, cell wall integrity and protein secretion. The impact on secretion was strongest in the case of ΔaslA and ΔaslB that showed increased pepsin, cellulase, and amylase activity. Biomass was reduced of agar cultures of ΔabaA, ΔaslA, ΔnosA, and ΔsrbA, while biomass was higher in liquid shaken cultures of ΔaslA and ΔaslB. The ΔaslA and ΔhtfA strains showed increased resistance to H2O2, while ΔaslB was more sensitive to this reactive oxygen species. Together, inactivation of the seven TF genes impacted biomass formation, sporulation, protein secretion, and stress resistance, and thereby these genes explain at least part of the pleiotropic phenotype of ΔflbA of A. niger.

AB - Inactivation of flbA in Aspergillus niger results in thinner cell walls, increased cell lysis, abolished sporulation, and an increased secretome complexity. A total of 36 transcription factor (TF) genes are differentially expressed in ΔflbA. Here, seven of these genes (abaA, aslA, aslB, azf1, htfA, nosA, and srbA) were inactivated. Inactivation of each of these genes affected sporulation and, with the exception of abaA, cell wall integrity and protein secretion. The impact on secretion was strongest in the case of ΔaslA and ΔaslB that showed increased pepsin, cellulase, and amylase activity. Biomass was reduced of agar cultures of ΔabaA, ΔaslA, ΔnosA, and ΔsrbA, while biomass was higher in liquid shaken cultures of ΔaslA and ΔaslB. The ΔaslA and ΔhtfA strains showed increased resistance to H2O2, while ΔaslB was more sensitive to this reactive oxygen species. Together, inactivation of the seven TF genes impacted biomass formation, sporulation, protein secretion, and stress resistance, and thereby these genes explain at least part of the pleiotropic phenotype of ΔflbA of A. niger.

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KW - Cell wall stress

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KW - Secretion

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The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors

Abstract

Bibliographical note

Funding

Keywords

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