The peplomer protein sequence of the M41 strain of coronavirus IBV and its comparison with Beaudette strains

H. G M Niesters; J. A. Lenstra; W. J M Spaan; A. J. Zijderveld; N. M C Bleumink-Pluym; F. Hong; G. J M van Scharrenburg; M. C. Horzinek; B. A M van der Zeijst

doi:10.1016/0168-1702(86)90022-5

The peplomer protein sequence of the M41 strain of coronavirus IBV and its comparison with Beaudette strains

H. G M Niesters^*, J. A. Lenstra, W. J M Spaan, A. J. Zijderveld, N. M C Bleumink-Pluym, F. Hong, G. J M van Scharrenburg, M. C. Horzinek, B. A M van der Zeijst

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The amino acid sequence of the gene for the peplomer protein of the vaccine strain M41 and the Beaudette laboratory strain M42-Salk of avian infectious bronchitis virus (IBV) have been derived from cDNA sequences. As found with other coronaviruses, the peplomer protein carries the epitopes eliciting neutralizing antibodies. The gene encodes a primary translation product of 1162 amino acids with a molecular weight of 128079. The use of a recent algorithm to predict membrane-protein interactions led to the unambiguous localization of the signah peptide and a transmembrane anchor α-helix at the C-terminus. At 50 positions amino acid differences were found between M41 and two Beaudette strains (M42-Salk and M42-Houghton). They are partly clustered in two regions of the protein. These two regions are candidates for neutralization epitopes of the protein.

Original language	English
Pages (from-to)	253-263
Number of pages	11
Journal	Virus Research
Volume	5
Issue number	2-3
DOIs	https://doi.org/10.1016/0168-1702(86)90022-5
Publication status	Published - 1 Jan 1986

Keywords

coronavirus IBV
M42
peplomer protein
strain M41

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0168-1702(86)90022-5

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Niesters, H. G. M., Lenstra, J. A., Spaan, W. J. M., Zijderveld, A. J., Bleumink-Pluym, N. M. C., Hong, F., van Scharrenburg, G. J. M., Horzinek, M. C., & van der Zeijst, B. A. M. (1986). The peplomer protein sequence of the M41 strain of coronavirus IBV and its comparison with Beaudette strains. Virus Research, 5(2-3), 253-263. https://doi.org/10.1016/0168-1702(86)90022-5

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abstract = "The amino acid sequence of the gene for the peplomer protein of the vaccine strain M41 and the Beaudette laboratory strain M42-Salk of avian infectious bronchitis virus (IBV) have been derived from cDNA sequences. As found with other coronaviruses, the peplomer protein carries the epitopes eliciting neutralizing antibodies. The gene encodes a primary translation product of 1162 amino acids with a molecular weight of 128079. The use of a recent algorithm to predict membrane-protein interactions led to the unambiguous localization of the signah peptide and a transmembrane anchor α-helix at the C-terminus. At 50 positions amino acid differences were found between M41 and two Beaudette strains (M42-Salk and M42-Houghton). They are partly clustered in two regions of the protein. These two regions are candidates for neutralization epitopes of the protein.",

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AU - Zijderveld, A. J.

AU - Bleumink-Pluym, N. M C

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AU - Horzinek, M. C.

AU - van der Zeijst, B. A M

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AB - The amino acid sequence of the gene for the peplomer protein of the vaccine strain M41 and the Beaudette laboratory strain M42-Salk of avian infectious bronchitis virus (IBV) have been derived from cDNA sequences. As found with other coronaviruses, the peplomer protein carries the epitopes eliciting neutralizing antibodies. The gene encodes a primary translation product of 1162 amino acids with a molecular weight of 128079. The use of a recent algorithm to predict membrane-protein interactions led to the unambiguous localization of the signah peptide and a transmembrane anchor α-helix at the C-terminus. At 50 positions amino acid differences were found between M41 and two Beaudette strains (M42-Salk and M42-Houghton). They are partly clustered in two regions of the protein. These two regions are candidates for neutralization epitopes of the protein.

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The peplomer protein sequence of the M41 strain of coronavirus IBV and its comparison with Beaudette strains

Abstract

Keywords

UN SDGs

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