TY - JOUR
T1 - The influence of pretreatment methods on saccharification of sugarcane bagasse by an enzyme extract from Chrysoporthe cubensis and commercial cocktails
T2 - A comparative study
AU - Maitan-Alfenas, Gabriela Piccolo
AU - Visser, Evan Michael
AU - Alfenas, Rafael Ferreira
AU - Nogueira, Bráulio Ris G
AU - de Campos, Guilherme Galvão
AU - Milagres, Adriane Ferreira
AU - de Vries, Ronald P.
AU - Guimarães, Valéria Monteze
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Biomass enzymatic hydrolysis depends on the pretreatment methods employed, the composition of initial feedstock and the enzyme cocktail used to release sugars for subsequent fermentation into ethanol. In this study, sugarcane bagasse was pretreated with 1% H2SO4 and 1% NaOH and the biomass saccharification was performed with 8% solids loading using 10 FPase units/g of bagasse of the enzymatic extract from Chrysoporthe cubensis and three commercial cocktails for a comparative study. Overall, the best glucose and xylose release was obtained from alkaline pretreated sugarcane bagasse. The C. cubensis extract promoted higher release of glucose (5.32g/L) and xylose (9.00g/L) than the commercial mixtures. Moreover, the C. cubensis extract presented high specific enzyme activities when compared to commercial cocktails mainly concerning to endoglucanase (331.84U/mg of protein), β-glucosidase (29.48U/mg of protein), β-xylosidase (2.95U/mg of protein), pectinase (127.46U/mg of protein) and laccase (2.49U/mg of protein).
AB - Biomass enzymatic hydrolysis depends on the pretreatment methods employed, the composition of initial feedstock and the enzyme cocktail used to release sugars for subsequent fermentation into ethanol. In this study, sugarcane bagasse was pretreated with 1% H2SO4 and 1% NaOH and the biomass saccharification was performed with 8% solids loading using 10 FPase units/g of bagasse of the enzymatic extract from Chrysoporthe cubensis and three commercial cocktails for a comparative study. Overall, the best glucose and xylose release was obtained from alkaline pretreated sugarcane bagasse. The C. cubensis extract promoted higher release of glucose (5.32g/L) and xylose (9.00g/L) than the commercial mixtures. Moreover, the C. cubensis extract presented high specific enzyme activities when compared to commercial cocktails mainly concerning to endoglucanase (331.84U/mg of protein), β-glucosidase (29.48U/mg of protein), β-xylosidase (2.95U/mg of protein), pectinase (127.46U/mg of protein) and laccase (2.49U/mg of protein).
KW - Biomass pretreatment
KW - Chrysoporthe cubensis
KW - Enzyme mixtures
KW - Saccharification
UR - http://www.scopus.com/inward/record.url?scp=84934918338&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2015.05.109
DO - 10.1016/j.biortech.2015.05.109
M3 - Article
AN - SCOPUS:84934918338
SN - 0960-8524
VL - 192
SP - 670
EP - 676
JO - Bioresource Technology
JF - Bioresource Technology
ER -