The immunologically reactive part of immunopurified circulating anodic antigen from Schistosoma mansoni is a threonine-linked polysaccharide consisting of →6)-(β-D-GlcpA-(1→3))-β-D-GalpNAc-(1→ repeating units

A.A. Bergwerff, G.J. Van Dam, J.P. Rotmans, A.M. Deelder, J.P. Kamerling, J.F.G. Vliegenthart

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The gut-associated excretory antigen CAA (circulating anodic antigen) from adult Schistosoma mansoni worms was isolated by immunoaffinity chromatography. Amino acid analysis following alkaline borohydride treatment indicated that CAA is a glycoprotein, O-glycosylated at Thr. The primary structure of the released O-glycan moiety was investigated by one- and two- dimensional, homo- and heteronuclear 1H and 13C NMR spectroscopy. It was found that the major carbohydrate chains have a novel polysaccharide structure, consisting of a branched disaccharide repeating unit containing 2- acetamido-2-deoxy-β-D-galactopyranose (β-D-GalpNAc) and β-D- glucopyranuronic acid (β-D-GlcpA). (→6)-β-D-GalpNAc-(1→)(n)3↑1β-D- GlcpA. The major antigenic character of CAA arises from this novel polysaccharide, which was shown to be an absolutely specific diagnostic marker in schistosomiasis. The cross-reactivity of CAA with anti-CCA (circulating cathodic antigen) monoclonal antibodies is caused by the presence of a small amount of O-linked CCA-poly-Lewis x carbohydrate chains on the CAA protein chain.
Original languageEnglish
Pages (from-to)31510-31517
Number of pages8
JournalJournal of Biological Chemistry
Volume269
Issue number50
Publication statusPublished - 12 Jul 1994

Keywords

  • antigen
  • polysaccharide
  • antigen detection
  • article
  • carbohydrate analysis
  • carbon nuclear magnetic resonance
  • immunogenicity
  • nonhuman
  • priority journal
  • proton nuclear magnetic resonance
  • Schistosoma mansoni
  • schistosomiasis
  • structure analysis

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