The HAUS Complex Is a Key Regulator of Non-centrosomal Microtubule Organization during Neuronal Development

Inês Cunha-Ferreira; Anaël Chazeau; Robin R Buijs; Riccardo Stucchi; Lena Will; Xingxiu Pan; Youri Adolfs; Christiaan van der Meer; Joanna C Wolthuis; Olga I Kahn; Philipp Schätzle; Maarten Altelaar; R Jeroen Pasterkamp; Lukas C Kapitein; Casper C Hoogenraad

doi:10.1016/j.celrep.2018.06.093

The HAUS Complex Is a Key Regulator of Non-centrosomal Microtubule Organization during Neuronal Development

Inês Cunha-Ferreira, Anaël Chazeau, Robin R Buijs, Riccardo Stucchi, Lena Will, Xingxiu Pan, Youri Adolfs, Christiaan van der Meer, Joanna C Wolthuis, Olga I Kahn, Philipp Schätzle, Maarten Altelaar, R Jeroen Pasterkamp, Lukas C Kapitein, Casper C Hoogenraad

University Medical Center Utrecht
Cell Biology, Department of Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, the Netherlands. Electronic address: [email protected].

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Neuron morphology and function are highly dependent on proper organization of the cytoskeleton. In neurons, the centrosome is inactivated early in development, and acentrosomal microtubules are generated by mechanisms that are poorly understood. Here, we show that neuronal migration, development, and polarization depend on the multi-subunit protein HAUS/augmin complex, previously described to be required for mitotic spindle assembly in dividing cells. The HAUS complex is essential for neuronal microtubule organization by ensuring uniform microtubule polarity in axons and regulation of microtubule density in dendrites. Using live-cell imaging and high-resolution microscopy, we found that distinct HAUS clusters are distributed throughout neurons and colocalize with γ-TuRC, suggesting local microtubule nucleation events. We propose that the HAUS complex locally regulates microtubule nucleation events to control proper neuronal development.

Original language	English
Pages (from-to)	791-800
Number of pages	10
Journal	Cell Reports
Volume	24
Issue number	4
DOIs	https://doi.org/10.1016/j.celrep.2018.06.093
Publication status	Published - 24 Jul 2018

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10.1016/j.celrep.2018.06.093

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Cunha-Ferreira, I., Chazeau, A., Buijs, R. R., Stucchi, R., Will, L., Pan, X., Adolfs, Y., van der Meer, C., Wolthuis, J. C., Kahn, O. I., Schätzle, P., Altelaar, M., Pasterkamp, R. J., Kapitein, L. C., & Hoogenraad, C. C. (2018). The HAUS Complex Is a Key Regulator of Non-centrosomal Microtubule Organization during Neuronal Development. Cell Reports, 24(4), 791-800. https://doi.org/10.1016/j.celrep.2018.06.093

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title = "The HAUS Complex Is a Key Regulator of Non-centrosomal Microtubule Organization during Neuronal Development",

abstract = "Neuron morphology and function are highly dependent on proper organization of the cytoskeleton. In neurons, the centrosome is inactivated early in development, and acentrosomal microtubules are generated by mechanisms that are poorly understood. Here, we show that neuronal migration, development, and polarization depend on the multi-subunit protein HAUS/augmin complex, previously described to be required for mitotic spindle assembly in dividing cells. The HAUS complex is essential for neuronal microtubule organization by ensuring uniform microtubule polarity in axons and regulation of microtubule density in dendrites. Using live-cell imaging and high-resolution microscopy, we found that distinct HAUS clusters are distributed throughout neurons and colocalize with γ-TuRC, suggesting local microtubule nucleation events. We propose that the HAUS complex locally regulates microtubule nucleation events to control proper neuronal development.",

author = "In{\^e}s Cunha-Ferreira and Ana{\"e}l Chazeau and Buijs, {Robin R} and Riccardo Stucchi and Lena Will and Xingxiu Pan and Youri Adolfs and {van der Meer}, Christiaan and Wolthuis, {Joanna C} and Kahn, {Olga I} and Philipp Sch{\"a}tzle and Maarten Altelaar and Pasterkamp, {R Jeroen} and Kapitein, {Lukas C} and Hoogenraad, {Casper C}",

year = "2018",

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doi = "10.1016/j.celrep.2018.06.093",

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Cunha-Ferreira, I, Chazeau, A, Buijs, RR, Stucchi, R, Will, L, Pan, X, Adolfs, Y, van der Meer, C, Wolthuis, JC, Kahn, OI, Schätzle, P, Altelaar, M, Pasterkamp, RJ, Kapitein, LC & Hoogenraad, CC 2018, 'The HAUS Complex Is a Key Regulator of Non-centrosomal Microtubule Organization during Neuronal Development', Cell Reports, vol. 24, no. 4, pp. 791-800. https://doi.org/10.1016/j.celrep.2018.06.093

TY - JOUR

T1 - The HAUS Complex Is a Key Regulator of Non-centrosomal Microtubule Organization during Neuronal Development

AU - Cunha-Ferreira, Inês

AU - Chazeau, Anaël

AU - Buijs, Robin R

AU - Stucchi, Riccardo

AU - Will, Lena

AU - Pan, Xingxiu

AU - Adolfs, Youri

AU - van der Meer, Christiaan

AU - Wolthuis, Joanna C

AU - Kahn, Olga I

AU - Schätzle, Philipp

AU - Altelaar, Maarten

AU - Pasterkamp, R Jeroen

AU - Kapitein, Lukas C

AU - Hoogenraad, Casper C

PY - 2018/7/24

Y1 - 2018/7/24

N2 - Neuron morphology and function are highly dependent on proper organization of the cytoskeleton. In neurons, the centrosome is inactivated early in development, and acentrosomal microtubules are generated by mechanisms that are poorly understood. Here, we show that neuronal migration, development, and polarization depend on the multi-subunit protein HAUS/augmin complex, previously described to be required for mitotic spindle assembly in dividing cells. The HAUS complex is essential for neuronal microtubule organization by ensuring uniform microtubule polarity in axons and regulation of microtubule density in dendrites. Using live-cell imaging and high-resolution microscopy, we found that distinct HAUS clusters are distributed throughout neurons and colocalize with γ-TuRC, suggesting local microtubule nucleation events. We propose that the HAUS complex locally regulates microtubule nucleation events to control proper neuronal development.

AB - Neuron morphology and function are highly dependent on proper organization of the cytoskeleton. In neurons, the centrosome is inactivated early in development, and acentrosomal microtubules are generated by mechanisms that are poorly understood. Here, we show that neuronal migration, development, and polarization depend on the multi-subunit protein HAUS/augmin complex, previously described to be required for mitotic spindle assembly in dividing cells. The HAUS complex is essential for neuronal microtubule organization by ensuring uniform microtubule polarity in axons and regulation of microtubule density in dendrites. Using live-cell imaging and high-resolution microscopy, we found that distinct HAUS clusters are distributed throughout neurons and colocalize with γ-TuRC, suggesting local microtubule nucleation events. We propose that the HAUS complex locally regulates microtubule nucleation events to control proper neuronal development.

U2 - 10.1016/j.celrep.2018.06.093

DO - 10.1016/j.celrep.2018.06.093

M3 - Article

C2 - 30044976

SN - 2211-1247

VL - 24

SP - 791

EP - 800

JO - Cell Reports

JF - Cell Reports

IS - 4

ER -

The HAUS Complex Is a Key Regulator of Non-centrosomal Microtubule Organization during Neuronal Development

Abstract

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