The En Tibi herbarium, a 16th century Italian treasure

‘Here for you a smiling garden of everlasting flowers’ is the inscription in Latin of a 16th century Italian herbarium kept in Leiden, the Netherlands, since 1690. The origin and botanical content of this herbarium, one of the oldest in existence today, have remained largely unknown. Here we present the plants included in this so-called ‘En Tibi’ herbarium, which comprises 473 specimens (455 taxa, 97 families), and discuss the geographical provenance of the book based on certain plant traits. The En Tibi is of great historical value as it contains some of the earliest herbarium records of numerous species, among which are useful plants such as oregano, thyme, tomato and hot pepper. Although prepared as a present, the En Tibi is a fine example of new botanical trends that arose in 16th century Italy. It is an attempt to reconstruct the herbals of classical authors such as Dioscorides, Theophrastus and Pliny, not with illustrations but with actual plant individuals. More than just a collection of medicinal plants, the En Tibi shows an emerging interest in the study of taxonomy and the discovery of new plants, unknown to classical authors. Analysis of the intrinsic and extrinsic traits of the plants reveals a temperate-Mediterranean origin, suggesting that the book was made in central or north–central Italy. Our botanical identification is the first necessary step to further elucidate the origin of the En Tibi and trace the mysterious compiler of this magnificent collection.