The effect of PEGT/PBT scaffold architecture on oxygen gradients in tissue engineered cartilaginous constructs

J Malda, T B F Woodfield, F van der Vloodt, F K Kooy, D E Martens, J Tramper, C A van Blitterswijk, J Riesle

Research output: Contribution to journalArticleAcademicpeer-review


Repair of articular cartilage defects using tissue engineered constructs composed of a scaffold and cultured autologous cells holds promise for future treatments. However, nutrient limitation (e.g. oxygen) has been suggested as a cause of the onset of chondrogenesis solely within the peripheral boundaries of larger constructs. In the present study, oxygen gradients were evaluated by microelectrode measurements in two porous polyethylene glycol terephthalate/polybutylene terephthalate (PEGT/PBT) scaffold architectures, a compression-molded and particle-leached sponge (CM) and a 3D-deposited fiber (3DF) scaffold. During the first 14 days in vitro, gradients intensified, after which a gradual decrease of the gradients was observed in vitro. In vivo, however, gradients changed instantly and became less pronounced. Although similar gradients were observed regardless of scaffold type, significantly more cells were present in the center of 3DF constructs after 2 weeks of in vivo culture. Our results stress the importance of a rationally designed scaffold for tissue-engineering applications. Organized structures, such as the 3DF PEGT/PBT polymer scaffolds, offer possibilities for regulation of nutrient supply and, therefore, hold promise for clinical approaches for cartilage repair.

Original languageEnglish
Pages (from-to)5773-80
Number of pages8
Issue number26
Publication statusPublished - Nov 2004


  • Animals
  • Biocompatible Materials/chemistry
  • Cartilage/cytology
  • Cattle
  • Cell Culture Techniques/methods
  • Cells, Cultured
  • Chondrocytes/cytology
  • Materials Testing
  • Mice
  • Mice, Nude
  • Molecular Conformation
  • Oxygen/analysis
  • Polyesters/analysis
  • Polyethylene Glycols/analysis
  • Surface Properties
  • Tissue Engineering/methods


Dive into the research topics of 'The effect of PEGT/PBT scaffold architecture on oxygen gradients in tissue engineered cartilaginous constructs'. Together they form a unique fingerprint.

Cite this