TY - JOUR
T1 - The Effect of Formulation and Processing Parameters on the Size of mPEG-b-p(HPMA-Bz) Polymeric Micelles
AU - Bagheri, Mahsa
AU - Bresseleers, Jaleesa
AU - Varela Moreira, Aida
AU - Sandre, Olivier
AU - Meeuwissen, Silvie A
AU - Schiffelers, Raymond M
AU - Metselaar, Josbert M
AU - van Nostrum, Cornelus F
AU - van Hest, Jan C M
AU - Hennink, Wim E
PY - 2018
Y1 - 2018
N2 - Micelles composed of block copolymers of poly(ethylene glycol)-b-poly(N-2-benzoyloxypropyl methacrylamide) (mPEG-b-p(HPMA-Bz)) have shown great promise as drug delivery carriers due to their excellent stability and high loading capacity. In the present study, parameters influencing micelle size were investigated to tailor sizes in the range of 25 to 100 nm. Micelles were prepared by a nanoprecipitation method and their size was modulated by the block copolymer properties such as molecular weight, their hydrophilic to hydrophobic ratio, homopolymer content, as well as formulation and processing parameters. It was shown that the micelles have a core-shell structure using a combination of dynamic light scattering and transmission electron microscopy analysis. By varying the degree of polymerization of the hydrophobic block (NB) between 68 and 10, at a fixed hydrophilic block mPEG5K (NA=114), it was shown that the hydrophobic core of the micelle was collapsed following the power law of (NB×Nagg)1/3. Further, the calculated brush height was similar for all the micelles examined (10 nm), indicating that crew-cut micelles were made. Both addition of homopolymer and preparation of micelles at lower concentrations or lower rates of addition of the organic solvent to the aqueous phase increased the size of micelles due to partitioning of the hydrophobic homopolymer chains to the core of the micelles and lower nucleation rates, respectively. Furthermore, it was shown that by using different solvents, the size of the micelles substantially changed. The use of acetone, acetonitrile, ethanol, THF and dioxane, resulted in micelles in the size range from 45 to 60 nm after removal of the organic solvents. The use of DMF and DMSO led to markedly larger sizes of 75 and 180 nm respectively. In conclusion, the results show that by modulating polymer properties and processing conditions, micelles with tailorable sizes can be obtained.
AB - Micelles composed of block copolymers of poly(ethylene glycol)-b-poly(N-2-benzoyloxypropyl methacrylamide) (mPEG-b-p(HPMA-Bz)) have shown great promise as drug delivery carriers due to their excellent stability and high loading capacity. In the present study, parameters influencing micelle size were investigated to tailor sizes in the range of 25 to 100 nm. Micelles were prepared by a nanoprecipitation method and their size was modulated by the block copolymer properties such as molecular weight, their hydrophilic to hydrophobic ratio, homopolymer content, as well as formulation and processing parameters. It was shown that the micelles have a core-shell structure using a combination of dynamic light scattering and transmission electron microscopy analysis. By varying the degree of polymerization of the hydrophobic block (NB) between 68 and 10, at a fixed hydrophilic block mPEG5K (NA=114), it was shown that the hydrophobic core of the micelle was collapsed following the power law of (NB×Nagg)1/3. Further, the calculated brush height was similar for all the micelles examined (10 nm), indicating that crew-cut micelles were made. Both addition of homopolymer and preparation of micelles at lower concentrations or lower rates of addition of the organic solvent to the aqueous phase increased the size of micelles due to partitioning of the hydrophobic homopolymer chains to the core of the micelles and lower nucleation rates, respectively. Furthermore, it was shown that by using different solvents, the size of the micelles substantially changed. The use of acetone, acetonitrile, ethanol, THF and dioxane, resulted in micelles in the size range from 45 to 60 nm after removal of the organic solvents. The use of DMF and DMSO led to markedly larger sizes of 75 and 180 nm respectively. In conclusion, the results show that by modulating polymer properties and processing conditions, micelles with tailorable sizes can be obtained.
U2 - 10.1021/acs.langmuir.8b03576
DO - 10.1021/acs.langmuir.8b03576
M3 - Article
C2 - 30415546
SN - 0743-7463
VL - 34
SP - 15495
EP - 15506
JO - Langmuir
JF - Langmuir
IS - 50
ER -