TY - JOUR
T1 - The diverse and expanding role of mass spectrometry in structural and molecular biology
AU - Lössl, Philip
AU - van de Waterbeemd, Michiel
AU - Heck, Albert Jr
N1 - © 2016 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
PY - 2016
Y1 - 2016
N2 - The emergence of proteomics has led to major technological advances in mass spectrometry (MS). These advancements not only benefitted MS-based high-throughput proteomics but also increased the impact of mass spectrometry on the field of structural and molecular biology. Here, we review how state-of-the-art MS methods, including native MS, top-down protein sequencing, cross-linking-MS, and hydrogen-deuterium exchange-MS, nowadays enable the characterization of biomolecular structures, functions, and interactions. In particular, we focus on the role of mass spectrometry in integrated structural and molecular biology investigations of biological macromolecular complexes and cellular machineries, highlighting work on CRISPR-Cas systems and eukaryotic transcription complexes.
AB - The emergence of proteomics has led to major technological advances in mass spectrometry (MS). These advancements not only benefitted MS-based high-throughput proteomics but also increased the impact of mass spectrometry on the field of structural and molecular biology. Here, we review how state-of-the-art MS methods, including native MS, top-down protein sequencing, cross-linking-MS, and hydrogen-deuterium exchange-MS, nowadays enable the characterization of biomolecular structures, functions, and interactions. In particular, we focus on the role of mass spectrometry in integrated structural and molecular biology investigations of biological macromolecular complexes and cellular machineries, highlighting work on CRISPR-Cas systems and eukaryotic transcription complexes.
U2 - 10.15252/embj.201694818
DO - 10.15252/embj.201694818
M3 - Article
C2 - 27797822
SN - 0261-4189
JO - EMBO Journal
JF - EMBO Journal
M1 - e201694818
ER -