The coronavirus nucleocapsid protein is dynamically associated with the replication-transcription complexes

Monique H Verheije; Marne C Hagemeijer; Mustafa Ulasli; Fulvio Reggiori; Peter J M Rottier; Paul S Masters; Cornelis A M de Haan

doi:10.1128/JVI.00569-10

The coronavirus nucleocapsid protein is dynamically associated with the replication-transcription complexes

Monique H Verheije, Marne C Hagemeijer, Mustafa Ulasli, Fulvio Reggiori, Peter J M Rottier, Paul S Masters, Cornelis A M de Haan

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The coronavirus nucleocapsid (N) protein is a virion structural protein. It also functions, however, in an unknown way in viral replication and localizes to the viral replication-transcription complexes (RTCs). Here we investigated, using recombinant murine coronaviruses expressing green fluorescent protein (GFP)-tagged versions of the N protein, the dynamics of its interactions with the RTCs and the domain(s) involved. Using fluorescent recovery after photobleaching, we showed that the N protein, unlike the nonstructural protein 2, is dynamically associated with the RTCs. Recruitment of the N protein to the RTCs requires the C-terminal N2b domain, which interacts with other N proteins in an RNA-independent manner.

Original language	English
Pages (from-to)	11575-11579
Number of pages	5
Journal	Journal of Virology
Volume	84
Issue number	21
DOIs	https://doi.org/10.1128/JVI.00569-10
Publication status	Published - Nov 2010

Keywords

Animals
Mice
Nucleocapsid Proteins
Protein Binding
Protein Structure, Tertiary
Protein Transport
Transcription, Genetic
Virus Replication

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10.1128/JVI.00569-10

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title = "The coronavirus nucleocapsid protein is dynamically associated with the replication-transcription complexes",

abstract = "The coronavirus nucleocapsid (N) protein is a virion structural protein. It also functions, however, in an unknown way in viral replication and localizes to the viral replication-transcription complexes (RTCs). Here we investigated, using recombinant murine coronaviruses expressing green fluorescent protein (GFP)-tagged versions of the N protein, the dynamics of its interactions with the RTCs and the domain(s) involved. Using fluorescent recovery after photobleaching, we showed that the N protein, unlike the nonstructural protein 2, is dynamically associated with the RTCs. Recruitment of the N protein to the RTCs requires the C-terminal N2b domain, which interacts with other N proteins in an RNA-independent manner.",

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author = "Verheije, {Monique H} and Hagemeijer, {Marne C} and Mustafa Ulasli and Fulvio Reggiori and Rottier, {Peter J M} and Masters, {Paul S} and {de Haan}, {Cornelis A M}",

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language = "English",

volume = "84",

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T1 - The coronavirus nucleocapsid protein is dynamically associated with the replication-transcription complexes

AU - Verheije, Monique H

AU - Hagemeijer, Marne C

AU - Ulasli, Mustafa

AU - Reggiori, Fulvio

AU - Rottier, Peter J M

AU - Masters, Paul S

AU - de Haan, Cornelis A M

PY - 2010/11

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N2 - The coronavirus nucleocapsid (N) protein is a virion structural protein. It also functions, however, in an unknown way in viral replication and localizes to the viral replication-transcription complexes (RTCs). Here we investigated, using recombinant murine coronaviruses expressing green fluorescent protein (GFP)-tagged versions of the N protein, the dynamics of its interactions with the RTCs and the domain(s) involved. Using fluorescent recovery after photobleaching, we showed that the N protein, unlike the nonstructural protein 2, is dynamically associated with the RTCs. Recruitment of the N protein to the RTCs requires the C-terminal N2b domain, which interacts with other N proteins in an RNA-independent manner.

AB - The coronavirus nucleocapsid (N) protein is a virion structural protein. It also functions, however, in an unknown way in viral replication and localizes to the viral replication-transcription complexes (RTCs). Here we investigated, using recombinant murine coronaviruses expressing green fluorescent protein (GFP)-tagged versions of the N protein, the dynamics of its interactions with the RTCs and the domain(s) involved. Using fluorescent recovery after photobleaching, we showed that the N protein, unlike the nonstructural protein 2, is dynamically associated with the RTCs. Recruitment of the N protein to the RTCs requires the C-terminal N2b domain, which interacts with other N proteins in an RNA-independent manner.

KW - Animals

KW - Mice

KW - Nucleocapsid Proteins

KW - Protein Binding

KW - Protein Structure, Tertiary

KW - Protein Transport

KW - Transcription, Genetic

KW - Virus Replication

U2 - 10.1128/JVI.00569-10

DO - 10.1128/JVI.00569-10

M3 - Article

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JO - Journal of Virology

JF - Journal of Virology

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ER -

The coronavirus nucleocapsid protein is dynamically associated with the replication-transcription complexes

Abstract

Keywords

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