The balance between plasmacytoid DC versus conventional DC determines pulmonary immunity to virus infections

Joost J Smit; Dennis M Lindell; Louis Boon; Mirjam Kool; Bart N Lambrecht; Nicholas W Lukacs

doi:10.1371/journal.pone.0001720

The balance between plasmacytoid DC versus conventional DC determines pulmonary immunity to virus infections

Joost J Smit, Dennis M Lindell, Louis Boon, Mirjam Kool, Bart N Lambrecht, Nicholas W Lukacs

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: Respiratory syncytial virus (RSV) infects nearly all infants by age 2 and is a leading cause of bronchiolitis. RSV may employ several mechanisms to induce immune dysregulation, including dendritic cell (DC) modulation during the immune response to RSV.

METHODS AND FINDINGS: Expansion of cDC and pDC by Flt3L treatment promoted an anti-viral response with reduced pathophysiology characterized by decreased airway hyperreactivity, reduced Th2 cytokines, increased Th1 cytokines, and a reduction in airway inflammation and mucus overexpression. These protective aspects of DC expansion could be completely reversed by depleting pDCs during the RSV infection. Expansion of DCs by Flt3L treatment enhanced in CD8+ T cell responses, which was reversed by depletion of pDC.

CONCLUSIONS: These results indicate that a balance between cDC and pDC in the lung and its lymph nodes is crucial for the outcome of a pulmonary infection. Increased pDC numbers induced by Flt3L treatment have a protective impact on the nature of the overall immune environment.

Original language	English
Pages (from-to)	e1720
Number of pages	9
Journal	PLoS One
Volume	3
Issue number	3
DOIs	https://doi.org/10.1371/journal.pone.0001720
Publication status	Published - 2008

Keywords

Animals
Bone Marrow
CD8-Positive T-Lymphocytes
Cytokines
Dendritic Cells
Female
Flow Cytometry
Humans
Infant
Lung
Lymph Nodes
Membrane Proteins
Mice
Mice, Inbred BALB C
Respiratory Syncytial Virus Infections
Respiratory Syncytial Virus, Human

Access to Document

10.1371/journal.pone.0001720

Cite this

@article{bca3f751af1a47fcbeb6420d17ac1b96,

title = "The balance between plasmacytoid DC versus conventional DC determines pulmonary immunity to virus infections",

abstract = "BACKGROUND: Respiratory syncytial virus (RSV) infects nearly all infants by age 2 and is a leading cause of bronchiolitis. RSV may employ several mechanisms to induce immune dysregulation, including dendritic cell (DC) modulation during the immune response to RSV.METHODS AND FINDINGS: Expansion of cDC and pDC by Flt3L treatment promoted an anti-viral response with reduced pathophysiology characterized by decreased airway hyperreactivity, reduced Th2 cytokines, increased Th1 cytokines, and a reduction in airway inflammation and mucus overexpression. These protective aspects of DC expansion could be completely reversed by depleting pDCs during the RSV infection. Expansion of DCs by Flt3L treatment enhanced in CD8+ T cell responses, which was reversed by depletion of pDC.CONCLUSIONS: These results indicate that a balance between cDC and pDC in the lung and its lymph nodes is crucial for the outcome of a pulmonary infection. Increased pDC numbers induced by Flt3L treatment have a protective impact on the nature of the overall immune environment.",

keywords = "Animals, Bone Marrow, CD8-Positive T-Lymphocytes, Cytokines, Dendritic Cells, Female, Flow Cytometry, Humans, Infant, Lung, Lymph Nodes, Membrane Proteins, Mice, Mice, Inbred BALB C, Respiratory Syncytial Virus Infections, Respiratory Syncytial Virus, Human",

author = "Smit, {Joost J} and Lindell, {Dennis M} and Louis Boon and Mirjam Kool and Lambrecht, {Bart N} and Lukacs, {Nicholas W}",

year = "2008",

doi = "10.1371/journal.pone.0001720",

language = "English",

volume = "3",

pages = "e1720",

journal = "PLoS One",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "3",

}

TY - JOUR

T1 - The balance between plasmacytoid DC versus conventional DC determines pulmonary immunity to virus infections

AU - Smit, Joost J

AU - Lindell, Dennis M

AU - Boon, Louis

AU - Kool, Mirjam

AU - Lambrecht, Bart N

AU - Lukacs, Nicholas W

PY - 2008

Y1 - 2008

N2 - BACKGROUND: Respiratory syncytial virus (RSV) infects nearly all infants by age 2 and is a leading cause of bronchiolitis. RSV may employ several mechanisms to induce immune dysregulation, including dendritic cell (DC) modulation during the immune response to RSV.METHODS AND FINDINGS: Expansion of cDC and pDC by Flt3L treatment promoted an anti-viral response with reduced pathophysiology characterized by decreased airway hyperreactivity, reduced Th2 cytokines, increased Th1 cytokines, and a reduction in airway inflammation and mucus overexpression. These protective aspects of DC expansion could be completely reversed by depleting pDCs during the RSV infection. Expansion of DCs by Flt3L treatment enhanced in CD8+ T cell responses, which was reversed by depletion of pDC.CONCLUSIONS: These results indicate that a balance between cDC and pDC in the lung and its lymph nodes is crucial for the outcome of a pulmonary infection. Increased pDC numbers induced by Flt3L treatment have a protective impact on the nature of the overall immune environment.

AB - BACKGROUND: Respiratory syncytial virus (RSV) infects nearly all infants by age 2 and is a leading cause of bronchiolitis. RSV may employ several mechanisms to induce immune dysregulation, including dendritic cell (DC) modulation during the immune response to RSV.METHODS AND FINDINGS: Expansion of cDC and pDC by Flt3L treatment promoted an anti-viral response with reduced pathophysiology characterized by decreased airway hyperreactivity, reduced Th2 cytokines, increased Th1 cytokines, and a reduction in airway inflammation and mucus overexpression. These protective aspects of DC expansion could be completely reversed by depleting pDCs during the RSV infection. Expansion of DCs by Flt3L treatment enhanced in CD8+ T cell responses, which was reversed by depletion of pDC.CONCLUSIONS: These results indicate that a balance between cDC and pDC in the lung and its lymph nodes is crucial for the outcome of a pulmonary infection. Increased pDC numbers induced by Flt3L treatment have a protective impact on the nature of the overall immune environment.

KW - Animals

KW - Bone Marrow

KW - CD8-Positive T-Lymphocytes

KW - Cytokines

KW - Dendritic Cells

KW - Female

KW - Flow Cytometry

KW - Humans

KW - Infant

KW - Lung

KW - Lymph Nodes

KW - Membrane Proteins

KW - Mice

KW - Mice, Inbred BALB C

KW - Respiratory Syncytial Virus Infections

KW - Respiratory Syncytial Virus, Human

U2 - 10.1371/journal.pone.0001720

DO - 10.1371/journal.pone.0001720

M3 - Article

C2 - 18320041

SN - 1932-6203

VL - 3

SP - e1720

JO - PLoS One

JF - PLoS One

IS - 3

ER -

The balance between plasmacytoid DC versus conventional DC determines pulmonary immunity to virus infections

Abstract

Keywords

Access to Document

Fingerprint

Cite this