The bacterial and fungal microbiome of the skin of healthy dogs and dogs with atopic dermatitis and the impact of topical antimicrobial therapy, an exploratory study

Suttiwee Chermprapai; Thomas H.A. Ederveen; Femke Broere; Els M. Broens; Yvette M. Schlotter; Saskia van Schalkwijk; Jos Boekhorst; Sacha A.F.T. van Hijum; Victor P.M.G. Rutten

doi:10.1016/j.vetmic.2018.12.022

The bacterial and fungal microbiome of the skin of healthy dogs and dogs with atopic dermatitis and the impact of topical antimicrobial therapy, an exploratory study

Suttiwee Chermprapai^*, Thomas H.A. Ederveen, Femke Broere, Els M. Broens, Yvette M. Schlotter, Saskia van Schalkwijk, Jos Boekhorst, Sacha A.F.T. van Hijum, Victor P.M.G. Rutten

^*Corresponding author for this work

Immunology

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Canine atopic dermatitis is a genetically predisposed inflammatory and pruritic allergic skin disease that is often complicated by (secondary) bacterial and fungal (yeast) infections. High-throughput DNA sequencing was used to characterize the composition of the microbiome (bacteria and fungi) inhabiting specific sites of skin in healthy dogs and dogs with atopic dermatitis (AD) before and after topical antimicrobial treatment. Skin microbiome samples were collected from six healthy control dogs and three dogs spontaneously affected by AD by swabbing at (non-) predilection sites before, during and after treatment. Bacteria and fungi were profiled by Illumina sequencing of the 16S ribosomal RNA gene of bacteria (16S) and the internally transcribed spacer of the ribosomal gene cassette in fungi (ITS). The total cohort of dogs showed a high diversity of microbes on skin with a strong individual variability of both 16S and ITS profiles. The genera of Staphylococcus and Porphyromonas were dominantly present both on atopic and healthy skin and across all skin sites studied. In addition, bacterial and fungal alpha diversity were similar at the different skin sites. The topical antimicrobial treatment increased the diversity of bacterial and fungal compositions in course of time on both AD and healthy skin.

Original language	English
Pages (from-to)	90-99
Number of pages	10
Journal	Veterinary Microbiology
Volume	229
DOIs	https://doi.org/10.1016/j.vetmic.2018.12.022
Publication status	Published - 1 Feb 2019

Funding

We would like to thank Patrick Zeeuwen for support in DNA sampling, isolation and amplification. This work was supported by a scholarship from the Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand ; and a ZonMw/NWO-ALW grant "Enabling Technologies" project number 40-43500-98-091. The funding sources had no involvement in study design; the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the article for publication. Appendix A

Keywords

16S
atopic dermatitis
canine
ITS
microbiome composition
topical treatment

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10.1016/j.vetmic.2018.12.022

1-s2.0-S0378113518311076-mainFinal published version, 6.54 MBLicence: CC BY-NC-ND

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Chermprapai, S., Ederveen, T. H. A., Broere, F., Broens, E. M., Schlotter, Y. M., van Schalkwijk, S., Boekhorst, J., van Hijum, S. A. F. T., & Rutten, V. P. M. G. (2019). The bacterial and fungal microbiome of the skin of healthy dogs and dogs with atopic dermatitis and the impact of topical antimicrobial therapy, an exploratory study. Veterinary Microbiology, 229, 90-99. https://doi.org/10.1016/j.vetmic.2018.12.022

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title = "The bacterial and fungal microbiome of the skin of healthy dogs and dogs with atopic dermatitis and the impact of topical antimicrobial therapy, an exploratory study",

abstract = "Canine atopic dermatitis is a genetically predisposed inflammatory and pruritic allergic skin disease that is often complicated by (secondary) bacterial and fungal (yeast) infections. High-throughput DNA sequencing was used to characterize the composition of the microbiome (bacteria and fungi) inhabiting specific sites of skin in healthy dogs and dogs with atopic dermatitis (AD) before and after topical antimicrobial treatment. Skin microbiome samples were collected from six healthy control dogs and three dogs spontaneously affected by AD by swabbing at (non-) predilection sites before, during and after treatment. Bacteria and fungi were profiled by Illumina sequencing of the 16S ribosomal RNA gene of bacteria (16S) and the internally transcribed spacer of the ribosomal gene cassette in fungi (ITS). The total cohort of dogs showed a high diversity of microbes on skin with a strong individual variability of both 16S and ITS profiles. The genera of Staphylococcus and Porphyromonas were dominantly present both on atopic and healthy skin and across all skin sites studied. In addition, bacterial and fungal alpha diversity were similar at the different skin sites. The topical antimicrobial treatment increased the diversity of bacterial and fungal compositions in course of time on both AD and healthy skin.",

keywords = "16S, atopic dermatitis, canine, ITS, microbiome composition, topical treatment",

author = "Suttiwee Chermprapai and Ederveen, \{Thomas H.A.\} and Femke Broere and Broens, \{Els M.\} and Schlotter, \{Yvette M.\} and \{van Schalkwijk\}, Saskia and Jos Boekhorst and \{van Hijum\}, \{Sacha A.F.T.\} and Rutten, \{Victor P.M.G.\}",

year = "2019",

month = feb,

day = "1",

doi = "10.1016/j.vetmic.2018.12.022",

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Chermprapai, S, Ederveen, THA, Broere, F , Broens, EM, Schlotter, YM, van Schalkwijk, S, Boekhorst, J, van Hijum, SAFT & Rutten, VPMG 2019, 'The bacterial and fungal microbiome of the skin of healthy dogs and dogs with atopic dermatitis and the impact of topical antimicrobial therapy, an exploratory study', Veterinary Microbiology, vol. 229, pp. 90-99. https://doi.org/10.1016/j.vetmic.2018.12.022

The bacterial and fungal microbiome of the skin of healthy dogs and dogs with atopic dermatitis and the impact of topical antimicrobial therapy, an exploratory study. / Chermprapai, Suttiwee; Ederveen, Thomas H.A.; Broere, Femke et al.
In: Veterinary Microbiology, Vol. 229, 01.02.2019, p. 90-99.

Research output: Contribution to journal › Article › Academic › peer-review

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T1 - The bacterial and fungal microbiome of the skin of healthy dogs and dogs with atopic dermatitis and the impact of topical antimicrobial therapy, an exploratory study

AU - Chermprapai, Suttiwee

AU - Ederveen, Thomas H.A.

AU - Broere, Femke

AU - Broens, Els M.

AU - Schlotter, Yvette M.

AU - van Schalkwijk, Saskia

AU - Boekhorst, Jos

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AU - Rutten, Victor P.M.G.

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AB - Canine atopic dermatitis is a genetically predisposed inflammatory and pruritic allergic skin disease that is often complicated by (secondary) bacterial and fungal (yeast) infections. High-throughput DNA sequencing was used to characterize the composition of the microbiome (bacteria and fungi) inhabiting specific sites of skin in healthy dogs and dogs with atopic dermatitis (AD) before and after topical antimicrobial treatment. Skin microbiome samples were collected from six healthy control dogs and three dogs spontaneously affected by AD by swabbing at (non-) predilection sites before, during and after treatment. Bacteria and fungi were profiled by Illumina sequencing of the 16S ribosomal RNA gene of bacteria (16S) and the internally transcribed spacer of the ribosomal gene cassette in fungi (ITS). The total cohort of dogs showed a high diversity of microbes on skin with a strong individual variability of both 16S and ITS profiles. The genera of Staphylococcus and Porphyromonas were dominantly present both on atopic and healthy skin and across all skin sites studied. In addition, bacterial and fungal alpha diversity were similar at the different skin sites. The topical antimicrobial treatment increased the diversity of bacterial and fungal compositions in course of time on both AD and healthy skin.

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KW - canine

KW - ITS

KW - microbiome composition

KW - topical treatment

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M3 - Article

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JO - Veterinary Microbiology

JF - Veterinary Microbiology

ER -

The bacterial and fungal microbiome of the skin of healthy dogs and dogs with atopic dermatitis and the impact of topical antimicrobial therapy, an exploratory study

Abstract

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Keywords

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