THE ASN-LINKED CARBOHYDRATE CHAINS OF HUMAN TAMM-HORSFALL GLYCOPROTEIN OF ONE MALE - NOVEL SULFATED AND NOVEL N-ACETYLGALACTOSAMINE-CONTAINING N-LINKED CARBOHYDRATE CHAINS

K HARD; G VAN ZADELHOFF; P MOONEN; Hans Kamerling; J Vliegenthart; Guus van Zadelhoff

THE ASN-LINKED CARBOHYDRATE CHAINS OF HUMAN TAMM-HORSFALL GLYCOPROTEIN OF ONE MALE - NOVEL SULFATED AND NOVEL N-ACETYLGALACTOSAMINE-CONTAINING N-LINKED CARBOHYDRATE CHAINS

K HARD, G VAN ZADELHOFF, P MOONEN, Hans Kamerling, J Vliegenthart, Guus van Zadelhoff

Utrecht University

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Human Tamm-Horsfall glycoprotein has been purified from the urine of one male. The Asn-linked carbohydrate chains were enzymically released by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F, and separated from the remaining protein by gel-permeation chromatography on Bio-Gel P-100. Fractionation of the intact (sulfated) sialylated carbohydrate chains was achieved by a combination of three liquid-chromatographic techniques, namely, anion-exchange FPLC on Q-Sepharose, amine-adsorption HPLC on Lichrospher-NH2, and high-pH anion-exchange chromatography on CarboPac PA1. In total, more than 150 carbohydrate-containing fractions were obtained, some of which still contained mixtures of oligosaccharides. The primary structure of 30 N-glycans, including 10 novel oligosaccharides, were determined by one- and two-dimensional H-1-NMR spectroscopy at 500 MHz or 600 MHz. The types of compounds identified range from non-fucosylated, monosialylated, diantennary to fucosylated, tetrasialylated, tetraantennary carbohydrate chains, possessing the following terminal structural elements:

[GRAPHICS]

The largest GalNAc-containing compound has the following structure:(~)[GRAPHICS]

Original language	English
Pages (from-to)	895-915
Number of pages	21
Journal	European Journal of Biochemistry
Volume	209
Issue number	3
Publication status	Published - 1 Nov 1992

Keywords

PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE-F
HAMSTER OVARY CELLS
STRUCTURAL-ANALYSIS
SIALIC-ACID
H-1-NMR SPECTROSCOPY
URINARY GLYCOPROTEIN
NMR-SPECTROSCOPY
OLIGOSACCHARIDES
IDENTIFICATION
UROMODULIN

Cite this

@article{c8f940e81d124cb29a046fe05882a0c5,

title = "THE ASN-LINKED CARBOHYDRATE CHAINS OF HUMAN TAMM-HORSFALL GLYCOPROTEIN OF ONE MALE - NOVEL SULFATED AND NOVEL N-ACETYLGALACTOSAMINE-CONTAINING N-LINKED CARBOHYDRATE CHAINS",

abstract = "Human Tamm-Horsfall glycoprotein has been purified from the urine of one male. The Asn-linked carbohydrate chains were enzymically released by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F, and separated from the remaining protein by gel-permeation chromatography on Bio-Gel P-100. Fractionation of the intact (sulfated) sialylated carbohydrate chains was achieved by a combination of three liquid-chromatographic techniques, namely, anion-exchange FPLC on Q-Sepharose, amine-adsorption HPLC on Lichrospher-NH2, and high-pH anion-exchange chromatography on CarboPac PA1. In total, more than 150 carbohydrate-containing fractions were obtained, some of which still contained mixtures of oligosaccharides. The primary structure of 30 N-glycans, including 10 novel oligosaccharides, were determined by one- and two-dimensional H-1-NMR spectroscopy at 500 MHz or 600 MHz. The types of compounds identified range from non-fucosylated, monosialylated, diantennary to fucosylated, tetrasialylated, tetraantennary carbohydrate chains, possessing the following terminal structural elements:[GRAPHICS]The largest GalNAc-containing compound has the following structure:(~)[GRAPHICS]",

keywords = "PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE-F, HAMSTER OVARY CELLS, STRUCTURAL-ANALYSIS, SIALIC-ACID, H-1-NMR SPECTROSCOPY, URINARY GLYCOPROTEIN, NMR-SPECTROSCOPY, OLIGOSACCHARIDES, IDENTIFICATION, UROMODULIN",

author = "K HARD and {VAN ZADELHOFF}, G and P MOONEN and Hans Kamerling and J Vliegenthart and {van Zadelhoff}, Guus",

year = "1992",

month = nov,

day = "1",

language = "English",

volume = "209",

pages = "895--915",

journal = "European Journal of Biochemistry",

issn = "0014-2956",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "3",

}

THE ASN-LINKED CARBOHYDRATE CHAINS OF HUMAN TAMM-HORSFALL GLYCOPROTEIN OF ONE MALE - NOVEL SULFATED AND NOVEL N-ACETYLGALACTOSAMINE-CONTAINING N-LINKED CARBOHYDRATE CHAINS. / HARD, K; VAN ZADELHOFF, G; MOONEN, P et al.
In: European Journal of Biochemistry, Vol. 209, No. 3, 01.11.1992, p. 895-915.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - THE ASN-LINKED CARBOHYDRATE CHAINS OF HUMAN TAMM-HORSFALL GLYCOPROTEIN OF ONE MALE - NOVEL SULFATED AND NOVEL N-ACETYLGALACTOSAMINE-CONTAINING N-LINKED CARBOHYDRATE CHAINS

AU - HARD, K

AU - VAN ZADELHOFF, G

AU - MOONEN, P

AU - Kamerling, Hans

AU - Vliegenthart, J

AU - van Zadelhoff, Guus

PY - 1992/11/1

Y1 - 1992/11/1

N2 - Human Tamm-Horsfall glycoprotein has been purified from the urine of one male. The Asn-linked carbohydrate chains were enzymically released by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F, and separated from the remaining protein by gel-permeation chromatography on Bio-Gel P-100. Fractionation of the intact (sulfated) sialylated carbohydrate chains was achieved by a combination of three liquid-chromatographic techniques, namely, anion-exchange FPLC on Q-Sepharose, amine-adsorption HPLC on Lichrospher-NH2, and high-pH anion-exchange chromatography on CarboPac PA1. In total, more than 150 carbohydrate-containing fractions were obtained, some of which still contained mixtures of oligosaccharides. The primary structure of 30 N-glycans, including 10 novel oligosaccharides, were determined by one- and two-dimensional H-1-NMR spectroscopy at 500 MHz or 600 MHz. The types of compounds identified range from non-fucosylated, monosialylated, diantennary to fucosylated, tetrasialylated, tetraantennary carbohydrate chains, possessing the following terminal structural elements:[GRAPHICS]The largest GalNAc-containing compound has the following structure:(~)[GRAPHICS]

AB - Human Tamm-Horsfall glycoprotein has been purified from the urine of one male. The Asn-linked carbohydrate chains were enzymically released by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F, and separated from the remaining protein by gel-permeation chromatography on Bio-Gel P-100. Fractionation of the intact (sulfated) sialylated carbohydrate chains was achieved by a combination of three liquid-chromatographic techniques, namely, anion-exchange FPLC on Q-Sepharose, amine-adsorption HPLC on Lichrospher-NH2, and high-pH anion-exchange chromatography on CarboPac PA1. In total, more than 150 carbohydrate-containing fractions were obtained, some of which still contained mixtures of oligosaccharides. The primary structure of 30 N-glycans, including 10 novel oligosaccharides, were determined by one- and two-dimensional H-1-NMR spectroscopy at 500 MHz or 600 MHz. The types of compounds identified range from non-fucosylated, monosialylated, diantennary to fucosylated, tetrasialylated, tetraantennary carbohydrate chains, possessing the following terminal structural elements:[GRAPHICS]The largest GalNAc-containing compound has the following structure:(~)[GRAPHICS]

KW - PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE-F

KW - HAMSTER OVARY CELLS

KW - STRUCTURAL-ANALYSIS

KW - SIALIC-ACID

KW - H-1-NMR SPECTROSCOPY

KW - URINARY GLYCOPROTEIN

KW - NMR-SPECTROSCOPY

KW - OLIGOSACCHARIDES

KW - IDENTIFICATION

KW - UROMODULIN

M3 - Article

SN - 0014-2956

VL - 209

SP - 895

EP - 915

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

IS - 3

ER -

THE ASN-LINKED CARBOHYDRATE CHAINS OF HUMAN TAMM-HORSFALL GLYCOPROTEIN OF ONE MALE - NOVEL SULFATED AND NOVEL N-ACETYLGALACTOSAMINE-CONTAINING N-LINKED CARBOHYDRATE CHAINS

Abstract

Keywords

Fingerprint

Cite this