Temperature Dependent AFM of Single Lipid and Mixed Lipid-Peptide Bilayers

F. Yarrow, J. P. J. M. van der Eerden, M. M. E. Snel

Research output: Contribution to journalArticleAcademicpeer-review


Regularly striped patterns are observed when imaging supported, mixed bilayers of the phospholipid DPPC and a synthetic α-helical transmembrane peptide (WALP) with contact mode AFM at ambient temperature. Temperature dependent AFM is used here as a tool to observe the temperature behavior of this system. Melting in a 2 mol% peptide/DPPC system starts at 36°C. The domains lie higher than the surrounding lipid bilayer at low temperatures (<36°C), but change to lower lying areas above that temperature. In contrast, pure lipid bilayers do not start melting until 40.5°C. Melting seems to start at defects present in the lipid bilayer.
Original languageEnglish
Pages (from-to)416-421
JournalAIP Conference Proceedings
Publication statusPublished - 1 Dec 2003


  • Lipids
  • Membranes bilayers and vesicles
  • Scanning tunneling and atomic force microscopy


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