TCR Bias and Affinity Define Two Compartments of the CD1b- Glycolipid-Specific T Cell Repertoire

Current views emphasize TCR diversity as a key feature that differentiates the group 1 (CD1a, CD1b, CD1c) and group 2 (CD1d)
CD1 systems. Whereas TCR sequence motifs define CD1d-reactive NKT cells, the available data do not allow a TCR-based organization
of the group 1 CD1 repertoire. The observed TCR diversity might result from donor-to-donor differences in TCR repertoire,
as seen for MHC-restricted T cells. Alternatively, diversity might result from differing CD1 isoforms, Ags, and methods used
to identify TCRs. Using CD1b tetramers to isolate clones recognizing the same glycolipid, we identified a previously unknown pattern
of V gene usage (TRAV17, TRBV4-1) among unrelated human subjects. These TCRs are distinct from those present on
NKT cells and germline-encoded mycolyl lipid–reactive T cells. Instead, they resemble the TCR of LDN5, one of the first known
CD1b-reactive clones that was previously thought to illustrate the diversity of the TCR repertoire. Interdonor TCR conservation
was observed in vitro and ex vivo, identifying LDN5-like T cells as a distinct T cell type. These data support TCR-based
organization of the CD1b repertoire, which consists of at least two compartments that differ in TCR sequence motifs, affinity,
and coreceptor expression.