T cell recognition of naturally presented epitopes of self-heat shock protein 70

Huib De Jong; Eva C. Koffeman; Jennifer M. Meerding; Rianne C. Scholman; Lotte Wieten; Wilco De Jager; Mark Klein; Henny Otten; Femke Van Wijk; Ruurd Van Der Zee; Johannes W J Bijlsma; Femke Broere; Willem Van Eden; Berent J. Prakken

doi:10.1007/s12192-013-0484-1

T cell recognition of naturally presented epitopes of self-heat shock protein 70

Huib De Jong, Eva C. Koffeman, Jennifer M. Meerding, Rianne C. Scholman, Lotte Wieten, Wilco De Jager, Mark Klein, Henny Otten, Femke Van Wijk, Ruurd Van Der Zee, Johannes W J Bijlsma, Femke Broere, Willem Van Eden, Berent J. Prakken

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Self-reactive T cells have shown to have a potential role as regulators of the immune system preventing or even suppressing autoimmunity. One of the most abundant proteins that can be eluted from human HLA molecules is heat shock protein 70 (HSP70). The aims of the current study are to identify HSP70 epitopes based on published HLA elution studies and to investigate whether T cells from healthy individuals may respond to such self-epitopes. A literature search and subsequent in silico binding prediction based on theoretical MHC binding motifs resulted in the identification of seven HSP70 epitopes. PBMCs of healthy controls proliferated after incubation with two of the seven peptides (H167 and H290). Furthermore H161, H290, and H443 induced CD69 expression or production of cytokines IFNγ or TNFα in healthy controls. The identification of these naturally presented epitopes and the response they elicit in the normal immune system make them potential candidates to study during inflammatory conditions as well as in autoimmune diseases.

Original language	English
Pages (from-to)	569-578
Number of pages	10
Journal	Cell Stress & Chaperones
Volume	19
Issue number	4
DOIs	https://doi.org/10.1007/s12192-013-0484-1
Publication status	Published - 15 Jan 2014

Keywords

Autoreactive T cells
Heat shock protein 70
HSP70
Human HSP70 peptides
Naturally processed T cell epitopes

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De Jong, H., Koffeman, E. C., Meerding, J. M., Scholman, R. C., Wieten, L., De Jager, W., Klein, M., Otten, H., Van Wijk, F., Van Der Zee, R., Bijlsma, J. W. J., Broere, F., Van Eden, W., & Prakken, B. J. (2014). T cell recognition of naturally presented epitopes of self-heat shock protein 70. Cell Stress & Chaperones, 19(4), 569-578. https://doi.org/10.1007/s12192-013-0484-1

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title = "T cell recognition of naturally presented epitopes of self-heat shock protein 70",

abstract = "Self-reactive T cells have shown to have a potential role as regulators of the immune system preventing or even suppressing autoimmunity. One of the most abundant proteins that can be eluted from human HLA molecules is heat shock protein 70 (HSP70). The aims of the current study are to identify HSP70 epitopes based on published HLA elution studies and to investigate whether T cells from healthy individuals may respond to such self-epitopes. A literature search and subsequent in silico binding prediction based on theoretical MHC binding motifs resulted in the identification of seven HSP70 epitopes. PBMCs of healthy controls proliferated after incubation with two of the seven peptides (H167 and H290). Furthermore H161, H290, and H443 induced CD69 expression or production of cytokines IFNγ or TNFα in healthy controls. The identification of these naturally presented epitopes and the response they elicit in the normal immune system make them potential candidates to study during inflammatory conditions as well as in autoimmune diseases.",

keywords = "Autoreactive T cells, Heat shock protein 70, HSP70, Human HSP70 peptides, Naturally processed T cell epitopes",

author = "{De Jong}, Huib and Koffeman, {Eva C.} and Meerding, {Jennifer M.} and Scholman, {Rianne C.} and Lotte Wieten and {De Jager}, Wilco and Mark Klein and Henny Otten and {Van Wijk}, Femke and {Van Der Zee}, Ruurd and Bijlsma, {Johannes W J} and Femke Broere and {Van Eden}, Willem and Prakken, {Berent J.}",

year = "2014",

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doi = "10.1007/s12192-013-0484-1",

language = "English",

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AU - De Jong, Huib

AU - Koffeman, Eva C.

AU - Meerding, Jennifer M.

AU - Scholman, Rianne C.

AU - Wieten, Lotte

AU - De Jager, Wilco

AU - Klein, Mark

AU - Otten, Henny

AU - Van Wijk, Femke

AU - Van Der Zee, Ruurd

AU - Bijlsma, Johannes W J

AU - Broere, Femke

AU - Van Eden, Willem

AU - Prakken, Berent J.

PY - 2014/1/15

Y1 - 2014/1/15

N2 - Self-reactive T cells have shown to have a potential role as regulators of the immune system preventing or even suppressing autoimmunity. One of the most abundant proteins that can be eluted from human HLA molecules is heat shock protein 70 (HSP70). The aims of the current study are to identify HSP70 epitopes based on published HLA elution studies and to investigate whether T cells from healthy individuals may respond to such self-epitopes. A literature search and subsequent in silico binding prediction based on theoretical MHC binding motifs resulted in the identification of seven HSP70 epitopes. PBMCs of healthy controls proliferated after incubation with two of the seven peptides (H167 and H290). Furthermore H161, H290, and H443 induced CD69 expression or production of cytokines IFNγ or TNFα in healthy controls. The identification of these naturally presented epitopes and the response they elicit in the normal immune system make them potential candidates to study during inflammatory conditions as well as in autoimmune diseases.

AB - Self-reactive T cells have shown to have a potential role as regulators of the immune system preventing or even suppressing autoimmunity. One of the most abundant proteins that can be eluted from human HLA molecules is heat shock protein 70 (HSP70). The aims of the current study are to identify HSP70 epitopes based on published HLA elution studies and to investigate whether T cells from healthy individuals may respond to such self-epitopes. A literature search and subsequent in silico binding prediction based on theoretical MHC binding motifs resulted in the identification of seven HSP70 epitopes. PBMCs of healthy controls proliferated after incubation with two of the seven peptides (H167 and H290). Furthermore H161, H290, and H443 induced CD69 expression or production of cytokines IFNγ or TNFα in healthy controls. The identification of these naturally presented epitopes and the response they elicit in the normal immune system make them potential candidates to study during inflammatory conditions as well as in autoimmune diseases.

KW - Autoreactive T cells

KW - Heat shock protein 70

KW - HSP70

KW - Human HSP70 peptides

KW - Naturally processed T cell epitopes

U2 - 10.1007/s12192-013-0484-1

DO - 10.1007/s12192-013-0484-1

M3 - Article

C2 - 24425585

SN - 1355-8145

VL - 19

SP - 569

EP - 578

JO - Cell Stress & Chaperones

JF - Cell Stress & Chaperones

IS - 4

ER -

T cell recognition of naturally presented epitopes of self-heat shock protein 70

Abstract

Keywords

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