Systematic antibody responses of calves to low molecular weight Cooperia oncophora antigens

M. G B Nieuwland; H. W. Ploeger; A. Kloosterman; H. K. Parmentier

doi:10.1016/0304-4017(94)00754-Z

Systematic antibody responses of calves to low molecular weight Cooperia oncophora antigens

M. G B Nieuwland, H. W. Ploeger, A. Kloosterman, H. K. Parmentier^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The systemic antibody responses to adult Cooperia oncophora antigen were studied using sera obtained from calves during a 6-week period following a single oral infection with either 20 000 or 100 000 third-stage C. oncophora larvae. Dose dependent increasing titres of IgG binding complete adult Cooperia antigen were found in the sera of Cooperia-infected calves. SDS-gel electrophoresis under reducing conditions, followed by Western blotting, revealed that the increase of IgG binding Cooperia antigens could be attributed mainly to specific binding of IgG to a complex of 12-15 kDa protein fragments of Cooperia adult antigen. This protein may represent a Cooperia oncophora-specific component that can be used for sero-diagnosis.

Original language	English
Pages (from-to)	231-239
Number of pages	9
Journal	Veterinary Parasitology
Volume	59
Issue number	3-4
DOIs	https://doi.org/10.1016/0304-4017(94)00754-Z
Publication status	Published - 1 Jan 1995

Keywords

Cattle-Nematoda
Cooperia oncophora
Humoral immunity
Proteins
Western blotting

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10.1016/0304-4017(94)00754-Z

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title = "Systematic antibody responses of calves to low molecular weight Cooperia oncophora antigens",

abstract = "The systemic antibody responses to adult Cooperia oncophora antigen were studied using sera obtained from calves during a 6-week period following a single oral infection with either 20 000 or 100 000 third-stage C. oncophora larvae. Dose dependent increasing titres of IgG binding complete adult Cooperia antigen were found in the sera of Cooperia-infected calves. SDS-gel electrophoresis under reducing conditions, followed by Western blotting, revealed that the increase of IgG binding Cooperia antigens could be attributed mainly to specific binding of IgG to a complex of 12-15 kDa protein fragments of Cooperia adult antigen. This protein may represent a Cooperia oncophora-specific component that can be used for sero-diagnosis.",

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AU - Nieuwland, M. G B

AU - Ploeger, H. W.

AU - Kloosterman, A.

AU - Parmentier, H. K.

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AB - The systemic antibody responses to adult Cooperia oncophora antigen were studied using sera obtained from calves during a 6-week period following a single oral infection with either 20 000 or 100 000 third-stage C. oncophora larvae. Dose dependent increasing titres of IgG binding complete adult Cooperia antigen were found in the sera of Cooperia-infected calves. SDS-gel electrophoresis under reducing conditions, followed by Western blotting, revealed that the increase of IgG binding Cooperia antigens could be attributed mainly to specific binding of IgG to a complex of 12-15 kDa protein fragments of Cooperia adult antigen. This protein may represent a Cooperia oncophora-specific component that can be used for sero-diagnosis.

KW - Cattle-Nematoda

KW - Cooperia oncophora

KW - Humoral immunity

KW - Proteins

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Systematic antibody responses of calves to low molecular weight Cooperia oncophora antigens

Abstract

Keywords

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