System-wide molecular evidence for phenotypic buffering in Arabidopsis

Jingyuan Fu; Joost J. B. Keurentjes; Harro Bouwmeester; Twan America; Francel W. A. Verstappen; Jane L. Ward; Michael H. Beale; Ric C. H. De Vos; Martijn Dijkstra; Richard A. Scheltema; Frank Johannes; Maarten Koornneef; Dick Vreugdenhil; Rainer Breitling; Ritsert C. Jansen

doi:10.1038/ng.308

System-wide molecular evidence for phenotypic buffering in Arabidopsis

Jingyuan Fu, Joost J. B. Keurentjes, Harro Bouwmeester, Twan America, Francel W. A. Verstappen, Jane L. Ward, Michael H. Beale, Ric C. H. De Vos, Martijn Dijkstra, Richard A. Scheltema, Frank Johannes, Maarten Koornneef, Dick Vreugdenhil, Rainer Breitling, Ritsert C. Jansen

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We profiled 162 lines of Arabidopsis for variation in transcript, protein and metabolite abundance using mRNA microarrays, two-dimensional polyacrylamide gel electrophoresis, gas chromatography time-of-flight mass spectrometry, liquid chromatography quadrupole time-of-flight mass spectrometry, and proton nuclear magnetic resonance. We added all publicly available phenotypic data from the same lines and mapped quantitative trait loci (QTL) for 40,580 molecular and 139 phenotypic traits. We found six QTL hot spots with major, system-wide effects, suggesting there are six breakpoints in a system otherwise buffered against many of the 500,000 SNPs. © 2009 Nature America, Inc. All rights reserved.

Original language	English
Pages (from-to)	166-167
Number of pages	2
Journal	Nature Genetics
Volume	41
Issue number	2
DOIs	https://doi.org/10.1038/ng.308
Publication status	Published - 1 Feb 2009
Externally published	Yes

Keywords

messenger RNA
protein
Arabidopsis
article
gas chromatography
gel electrophoresis
gene expression profiling
gene mapping
genetic transcription
genetic variability
liquid chromatography
metabolite
microarray analysis
nonhuman
phenotype
polyacrylamide gel electrophoresis
priority journal
proton nuclear magnetic resonance
quadrupole mass spectrometry
quantitative trait locus
single nucleotide polymorphism
time of flight mass spectrometry

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Fu, J., Keurentjes, J. J. B., Bouwmeester, H., America, T., Verstappen, F. W. A., Ward, J. L., Beale, M. H., De Vos, R. C. H., Dijkstra, M., Scheltema, R. A., Johannes, F., Koornneef, M., Vreugdenhil, D., Breitling, R., & Jansen, R. C. (2009). System-wide molecular evidence for phenotypic buffering in Arabidopsis. Nature Genetics, 41(2), 166-167. https://doi.org/10.1038/ng.308

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title = "System-wide molecular evidence for phenotypic buffering in Arabidopsis",

abstract = "We profiled 162 lines of Arabidopsis for variation in transcript, protein and metabolite abundance using mRNA microarrays, two-dimensional polyacrylamide gel electrophoresis, gas chromatography time-of-flight mass spectrometry, liquid chromatography quadrupole time-of-flight mass spectrometry, and proton nuclear magnetic resonance. We added all publicly available phenotypic data from the same lines and mapped quantitative trait loci (QTL) for 40,580 molecular and 139 phenotypic traits. We found six QTL hot spots with major, system-wide effects, suggesting there are six breakpoints in a system otherwise buffered against many of the 500,000 SNPs. {\textcopyright} 2009 Nature America, Inc. All rights reserved.",

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author = "Jingyuan Fu and Keurentjes, {Joost J. B.} and Harro Bouwmeester and Twan America and Verstappen, {Francel W. A.} and Ward, {Jane L.} and Beale, {Michael H.} and {De Vos}, {Ric C. H.} and Martijn Dijkstra and Scheltema, {Richard A.} and Frank Johannes and Maarten Koornneef and Dick Vreugdenhil and Rainer Breitling and Jansen, {Ritsert C.}",

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AU - Ward, Jane L.

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AU - Dijkstra, Martijn

AU - Scheltema, Richard A.

AU - Johannes, Frank

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AB - We profiled 162 lines of Arabidopsis for variation in transcript, protein and metabolite abundance using mRNA microarrays, two-dimensional polyacrylamide gel electrophoresis, gas chromatography time-of-flight mass spectrometry, liquid chromatography quadrupole time-of-flight mass spectrometry, and proton nuclear magnetic resonance. We added all publicly available phenotypic data from the same lines and mapped quantitative trait loci (QTL) for 40,580 molecular and 139 phenotypic traits. We found six QTL hot spots with major, system-wide effects, suggesting there are six breakpoints in a system otherwise buffered against many of the 500,000 SNPs. © 2009 Nature America, Inc. All rights reserved.

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System-wide molecular evidence for phenotypic buffering in Arabidopsis

Abstract

Keywords

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