TY - JOUR
T1 - Synthetic Extracellular Matrices as a Toolbox to Tune Stem Cell Secretome
AU - Liu, Kaizheng
AU - Veenendaal, Tomas
AU - Wiendels, Maury
AU - Ruiz-Zapata, Alejandra M
AU - van Laar, Justin
AU - Kyranas, Rafail
AU - Enting, Hilde
AU - van Cranenbroek, Bram
AU - Koenen, Hans J P M
AU - Mihaila, Silvia M
AU - Oosterwijk, Egbert
AU - Kouwer, Paul H J
PY - 2020/12/23
Y1 - 2020/12/23
N2 - The application of stem cell-derived secretome in regenerative therapies offers the key advantage that instead of the stem cells, only their effective paracrine compounds are in vivo delivered. Ideally, the secretome can be steered by the culture conditions of the stem cells. So far, most studies use stem cells cultured on stiff plastic substrates, not representative of their native 3D environment. In this study, cells are cultured inside synthetic polyisocyanide (PIC)-based hydrogels, which are minimal, tailorable, and highly reproducible biomimetic matrices. Secretome analysis of human adipose-derived stem cells (multiplex, ELISA) displays that matrix manipulation is a powerful tool to direct the secretome composition. As an example, cells in nonadherent PIC gels secrete increased levels of IL-10 and the conditioned media from 3D culture accelerate wound closure. In all, our PIC-based approach opens the door to dedicated matrix design to engineer the secretome for custom applications.
AB - The application of stem cell-derived secretome in regenerative therapies offers the key advantage that instead of the stem cells, only their effective paracrine compounds are in vivo delivered. Ideally, the secretome can be steered by the culture conditions of the stem cells. So far, most studies use stem cells cultured on stiff plastic substrates, not representative of their native 3D environment. In this study, cells are cultured inside synthetic polyisocyanide (PIC)-based hydrogels, which are minimal, tailorable, and highly reproducible biomimetic matrices. Secretome analysis of human adipose-derived stem cells (multiplex, ELISA) displays that matrix manipulation is a powerful tool to direct the secretome composition. As an example, cells in nonadherent PIC gels secrete increased levels of IL-10 and the conditioned media from 3D culture accelerate wound closure. In all, our PIC-based approach opens the door to dedicated matrix design to engineer the secretome for custom applications.
KW - synthetic extracellular matrices (ECM)
KW - stem cell secretome
KW - polyisocyanide hydrogels
KW - interleukin-10 (IL-10)
KW - wound healing
U2 - 10.1021/acsami.0c16208
DO - 10.1021/acsami.0c16208
M3 - Article
C2 - 33305561
SN - 1944-8244
VL - 12
SP - 56723
EP - 56730
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
IS - 51
ER -