SV40-transformed hamster lens epithelial cells: A novel system for the isolation of cytoskeletal messenger RNAs and their translation products

H. Bloemendal; J. A. Lenstra; H. Dodemont; F. C S Ramaekers; A. A. Groeneveld; I. Dunia; E. L. Benedetti

doi:10.1016/S0014-4835(80)80010-8

SV40-transformed hamster lens epithelial cells: A novel system for the isolation of cytoskeletal messenger RNAs and their translation products

H. Bloemendal^*, J. A. Lenstra, H. Dodemont, F. C S Ramaekers, A. A. Groeneveld, I. Dunia, E. L. Benedetti

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Epithelial cells from Syrian hamster lens were cultured and transformed by Simian Virus 40 (SV40). This cell line can be brought into suspension culture and then grows very efficiently. The quantitatively major proteins synthesized by these cells appeared to be the cytoskeletal constituents actin and vimentin. Morphologically the occurrence of the two proteins in the cultured cells is reflected by the presence of actin filaments and intermediate-sized filaments. This was demonstrated by immunofluorescence studies and electron microscopy. A messenger RNA fraction that was isolated from the transformed cells directed almost exclusively the synthesis of actin and vimentin after incubation in a heterologous cell-free system.

Original language	English
Pages (from-to)	513-525
Number of pages	13
Journal	Experimental Eye Research
Volume	31
Issue number	5
DOIs	https://doi.org/10.1016/S0014-4835(80)80010-8
Publication status	Published - 1 Jan 1980

Keywords

cytoskeletal mRNA
lens suspension culture
transformed lens cells

Access to Document

10.1016/S0014-4835(80)80010-8

Cite this

@article{b3f87fe10448457d95b2052f83a21f25,

title = "SV40-transformed hamster lens epithelial cells: A novel system for the isolation of cytoskeletal messenger RNAs and their translation products",

abstract = "Epithelial cells from Syrian hamster lens were cultured and transformed by Simian Virus 40 (SV40). This cell line can be brought into suspension culture and then grows very efficiently. The quantitatively major proteins synthesized by these cells appeared to be the cytoskeletal constituents actin and vimentin. Morphologically the occurrence of the two proteins in the cultured cells is reflected by the presence of actin filaments and intermediate-sized filaments. This was demonstrated by immunofluorescence studies and electron microscopy. A messenger RNA fraction that was isolated from the transformed cells directed almost exclusively the synthesis of actin and vimentin after incubation in a heterologous cell-free system.",

keywords = "cytoskeletal mRNA, lens suspension culture, transformed lens cells",

author = "H. Bloemendal and Lenstra, {J. A.} and H. Dodemont and Ramaekers, {F. C S} and Groeneveld, {A. A.} and I. Dunia and Benedetti, {E. L.}",

year = "1980",

month = jan,

day = "1",

doi = "10.1016/S0014-4835(80)80010-8",

language = "English",

volume = "31",

pages = "513--525",

journal = "Experimental Eye Research",

issn = "0014-4835",

publisher = "Academic Press",

number = "5",

}

TY - JOUR

T1 - SV40-transformed hamster lens epithelial cells

T2 - A novel system for the isolation of cytoskeletal messenger RNAs and their translation products

AU - Bloemendal, H.

AU - Lenstra, J. A.

AU - Dodemont, H.

AU - Ramaekers, F. C S

AU - Groeneveld, A. A.

AU - Dunia, I.

AU - Benedetti, E. L.

PY - 1980/1/1

Y1 - 1980/1/1

N2 - Epithelial cells from Syrian hamster lens were cultured and transformed by Simian Virus 40 (SV40). This cell line can be brought into suspension culture and then grows very efficiently. The quantitatively major proteins synthesized by these cells appeared to be the cytoskeletal constituents actin and vimentin. Morphologically the occurrence of the two proteins in the cultured cells is reflected by the presence of actin filaments and intermediate-sized filaments. This was demonstrated by immunofluorescence studies and electron microscopy. A messenger RNA fraction that was isolated from the transformed cells directed almost exclusively the synthesis of actin and vimentin after incubation in a heterologous cell-free system.

AB - Epithelial cells from Syrian hamster lens were cultured and transformed by Simian Virus 40 (SV40). This cell line can be brought into suspension culture and then grows very efficiently. The quantitatively major proteins synthesized by these cells appeared to be the cytoskeletal constituents actin and vimentin. Morphologically the occurrence of the two proteins in the cultured cells is reflected by the presence of actin filaments and intermediate-sized filaments. This was demonstrated by immunofluorescence studies and electron microscopy. A messenger RNA fraction that was isolated from the transformed cells directed almost exclusively the synthesis of actin and vimentin after incubation in a heterologous cell-free system.

KW - cytoskeletal mRNA

KW - lens suspension culture

KW - transformed lens cells

UR - http://www.scopus.com/inward/record.url?scp=0019142556&partnerID=8YFLogxK

U2 - 10.1016/S0014-4835(80)80010-8

DO - 10.1016/S0014-4835(80)80010-8

M3 - Article

C2 - 6256194

AN - SCOPUS:0019142556

SN - 0014-4835

VL - 31

SP - 513

EP - 525

JO - Experimental Eye Research

JF - Experimental Eye Research

IS - 5

ER -

SV40-transformed hamster lens epithelial cells: A novel system for the isolation of cytoskeletal messenger RNAs and their translation products

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this