Super-Resolution Imaging of Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic Therapy

Enzo M. Scutigliani; Jakub A. Kochan; Emilie C.B. Desclos; Art Jonker; Michal Heger; Przemek M. Krawczyk

doi:10.1007/978-1-0716-2099-1_37

Super-Resolution Imaging of Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic Therapy

Enzo M. Scutigliani, Jakub A. Kochan, Emilie C.B. Desclos, Art Jonker, Michal Heger, Przemek M. Krawczyk^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

Liposomal nanocarriers are intensively investigated as delivery vehicles for photoactivatable agents used in photodynamic therapy (PDT). The uptake, intracellular distribution, and processing of the nanocarriers are of paramount importance for the effectiveness of the therapy; visualization and analysis of these processes can, therefore, stimulate the development of improved PDT modalities. Here we describe a simple protocol, based on super-resolution imaging, that can be used for detailed quantification of concentration, distribution, and size of individual lipid nanocarriers in adherent mammalian cells.

Original language	English
Title of host publication	Photodynamic Therapy
Subtitle of host publication	Methods and Protocols
Editors	Mans Broekgaarden, Hong Zhang, Mladen Korbelik, Michael R. Hamblin, Michal Heger
Place of Publication	New York
Publisher	Humana Press
Pages	703-709
Number of pages	7
Edition	1
ISBN (Electronic)	978-1-0716-2099-1
ISBN (Print)	978-1-0716-2098-4, 978-1-0716-2101-1
DOIs	https://doi.org/10.1007/978-1-0716-2099-1_37
Publication status	Published - 4 May 2022

Publication series

Name	Methods in Molecular Biology
Publisher	Humana
Volume	2451
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Photodynamic therapy
Photosensitizers
Super-resolution microscopy

Access to Document

10.1007/978-1-0716-2099-1_37

978-1-0716-2099-1_37Final published version, 266 KBLicence: Taverne

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Scutigliani, E. M., Kochan, J. A., Desclos, E. C. B., Jonker, A., Heger, M., & Krawczyk, P. M. (2022). Super-Resolution Imaging of Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic Therapy. In M. Broekgaarden, H. Zhang, M. Korbelik, M. R. Hamblin, & M. Heger (Eds.), Photodynamic Therapy: Methods and Protocols (1 ed., pp. 703-709). (Methods in Molecular Biology; Vol. 2451). Humana Press. https://doi.org/10.1007/978-1-0716-2099-1_37

Scutigliani, Enzo M. ; Kochan, Jakub A. ; Desclos, Emilie C.B. et al. / Super-Resolution Imaging of Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic Therapy. Photodynamic Therapy: Methods and Protocols. editor / Mans Broekgaarden ; Hong Zhang ; Mladen Korbelik ; Michael R. Hamblin ; Michal Heger. 1. ed. New York : Humana Press, 2022. pp. 703-709 (Methods in Molecular Biology).

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abstract = "Liposomal nanocarriers are intensively investigated as delivery vehicles for photoactivatable agents used in photodynamic therapy (PDT). The uptake, intracellular distribution, and processing of the nanocarriers are of paramount importance for the effectiveness of the therapy; visualization and analysis of these processes can, therefore, stimulate the development of improved PDT modalities. Here we describe a simple protocol, based on super-resolution imaging, that can be used for detailed quantification of concentration, distribution, and size of individual lipid nanocarriers in adherent mammalian cells.",

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note = "Publisher Copyright: {\textcopyright} 2022, Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

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Scutigliani, EM, Kochan, JA, Desclos, ECB, Jonker, A, Heger, M & Krawczyk, PM 2022, Super-Resolution Imaging of Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic Therapy. in M Broekgaarden, H Zhang, M Korbelik, MR Hamblin & M Heger (eds), Photodynamic Therapy: Methods and Protocols. 1 edn, Methods in Molecular Biology, vol. 2451, Humana Press, New York, pp. 703-709. https://doi.org/10.1007/978-1-0716-2099-1_37

Super-Resolution Imaging of Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic Therapy. / Scutigliani, Enzo M.; Kochan, Jakub A.; Desclos, Emilie C.B. et al.
Photodynamic Therapy: Methods and Protocols. ed. / Mans Broekgaarden; Hong Zhang; Mladen Korbelik; Michael R. Hamblin; Michal Heger. 1. ed. New York: Humana Press, 2022. p. 703-709 (Methods in Molecular Biology; Vol. 2451).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

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T1 - Super-Resolution Imaging of Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic Therapy

AU - Scutigliani, Enzo M.

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AU - Jonker, Art

AU - Heger, Michal

AU - Krawczyk, Przemek M.

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KW - Super-resolution microscopy

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Scutigliani EM, Kochan JA, Desclos ECB, Jonker A, Heger M, Krawczyk PM. Super-Resolution Imaging of Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic Therapy. In Broekgaarden M, Zhang H, Korbelik M, Hamblin MR, Heger M, editors, Photodynamic Therapy: Methods and Protocols. 1 ed. New York: Humana Press. 2022. p. 703-709. (Methods in Molecular Biology). doi: 10.1007/978-1-0716-2099-1_37

Super-Resolution Imaging of Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic Therapy

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