Abstract
The 'sulfo-click' reaction, which is a chemoselective amidation reaction involving the reaction of an aminoethane sulfonyl azide with a thio acid, encompasses a new approach for ligation and conjugation. Detailed protocols are provided for decorating biologically active peptides or dendrimers with biophysical tags, fluorescent probes, metal chelators, and small peptides by using this reaction as a novel, metal-free 'sulfo-click' approach. Copyright © 2009 European Peptide Society and John Wiley & Sons, Ltd.
| Original language | English |
|---|---|
| Pages (from-to) | 1-5 |
| Number of pages | 5 |
| Journal | Journal of Peptide Science |
| Volume | 16 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2010 |
Keywords
- 'sulfo-click'
- Amidation reactions
- Bioconjugation
- Chemoselectivity
- Thio acids/sulfonyl azides
- tetraxetan
- amide
- aminoethanesulfonyl azide
- azide
- biotin derivative
- biotin thioacid derivative
- chelating agent
- coumarin derivative
- cysteine
- dendrimer
- ethylamine
- fluorescent dye
- lactoferrin
- n acyl sulfonamide
- peptide derivative
- peptidyl sulfonamide
- resin
- sulfonamide
- thioacetic acid
- unclassified drug
- addition reaction
- amidation
- article
- chemical structure
- conjugation
- crystallization
- human
- priority journal
- protein modification
- sulfo click reaction
- synthesis