Sulfated di-, tri- and tetraantennary N-glycans in human Tamm-Horsfall glycoprotein

Johannes J. M. Van Rooijen; Johannis P. Kamerling; Johannes F. G. Vliegenthart

doi:10.1046/j.1432-1327.1998.2560471.x

Sulfated di-, tri- and tetraantennary N-glycans in human Tamm-Horsfall glycoprotein

Johannes J. M. Van Rooijen
, Johannis P. Kamerling
, Johannes F. G. Vliegenthart

Sub NMR Spectroscopy

Bijvoet Center

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The primary structures of 32 sulfated di-, tri- and tetraantennary N-glycans of human Tamm-Horsfall glycoprotein (THP) have been determined. THP was isolated from the urine of one healthy male donor. Tile intact carbohydrate chains were released by PNGase-F and fractionated via FPLC on Resource Q, HPLC an LiChrosorb-NH2, and high-pH anion-exchange chromatography on CarboPac PA-1. Characterizations were performed using 500-MHz and 600-MHz 1H-NMR spectroscopy, in combination with sialidase treatments. The type of characterized N-glycans ranged from monosulfated to trisulfated N-glycans, whereby the sulfate groups were present as 3-O-sulfated Gal (Gal3S) and 4-O-sulfated GalNAc (GalNAc4S).

Original language	English
Pages (from-to)	471-487
Number of pages	17
Journal	European Journal of Biochemistry
Volume	256
Issue number	2
DOIs	https://doi.org/10.1046/j.1432-1327.1998.2560471.x
Publication status	Published - 9 Jul 1998

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Carbohydrate
NMR
Sulfated N-glycan
Tamm-Horsfall glycoprotein
Tamm Horsfall glycoprotein
article
carbohydrate analysis
controlled study
drug isolation
drug structure
high performance liquid chromatography
human
human tissue
normal human
nuclear magnetic resonance spectroscopy
priority journal
urine

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title = "Sulfated di-, tri- and tetraantennary N-glycans in human Tamm-Horsfall glycoprotein",

abstract = "The primary structures of 32 sulfated di-, tri- and tetraantennary N-glycans of human Tamm-Horsfall glycoprotein (THP) have been determined. THP was isolated from the urine of one healthy male donor. Tile intact carbohydrate chains were released by PNGase-F and fractionated via FPLC on Resource Q, HPLC an LiChrosorb-NH2, and high-pH anion-exchange chromatography on CarboPac PA-1. Characterizations were performed using 500-MHz and 600-MHz 1H-NMR spectroscopy, in combination with sialidase treatments. The type of characterized N-glycans ranged from monosulfated to trisulfated N-glycans, whereby the sulfate groups were present as 3-O-sulfated Gal (Gal3S) and 4-O-sulfated GalNAc (GalNAc4S).",

keywords = "Carbohydrate, NMR, Sulfated N-glycan, Tamm-Horsfall glycoprotein, Tamm Horsfall glycoprotein, article, carbohydrate analysis, controlled study, drug isolation, drug structure, high performance liquid chromatography, human, human tissue, normal human, nuclear magnetic resonance spectroscopy, priority journal, urine",

author = "\{Van Rooijen\}, \{Johannes J. M.\} and Kamerling, \{Johannis P.\} and Vliegenthart, \{Johannes F. G.\}",

year = "1998",

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doi = "10.1046/j.1432-1327.1998.2560471.x",

language = "English",

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pages = "471--487",

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T1 - Sulfated di-, tri- and tetraantennary N-glycans in human Tamm-Horsfall glycoprotein

AU - Van Rooijen, Johannes J. M.

AU - Kamerling, Johannis P.

AU - Vliegenthart, Johannes F. G.

PY - 1998/7/9

Y1 - 1998/7/9

N2 - The primary structures of 32 sulfated di-, tri- and tetraantennary N-glycans of human Tamm-Horsfall glycoprotein (THP) have been determined. THP was isolated from the urine of one healthy male donor. Tile intact carbohydrate chains were released by PNGase-F and fractionated via FPLC on Resource Q, HPLC an LiChrosorb-NH2, and high-pH anion-exchange chromatography on CarboPac PA-1. Characterizations were performed using 500-MHz and 600-MHz 1H-NMR spectroscopy, in combination with sialidase treatments. The type of characterized N-glycans ranged from monosulfated to trisulfated N-glycans, whereby the sulfate groups were present as 3-O-sulfated Gal (Gal3S) and 4-O-sulfated GalNAc (GalNAc4S).

AB - The primary structures of 32 sulfated di-, tri- and tetraantennary N-glycans of human Tamm-Horsfall glycoprotein (THP) have been determined. THP was isolated from the urine of one healthy male donor. Tile intact carbohydrate chains were released by PNGase-F and fractionated via FPLC on Resource Q, HPLC an LiChrosorb-NH2, and high-pH anion-exchange chromatography on CarboPac PA-1. Characterizations were performed using 500-MHz and 600-MHz 1H-NMR spectroscopy, in combination with sialidase treatments. The type of characterized N-glycans ranged from monosulfated to trisulfated N-glycans, whereby the sulfate groups were present as 3-O-sulfated Gal (Gal3S) and 4-O-sulfated GalNAc (GalNAc4S).

KW - Carbohydrate

KW - NMR

KW - Sulfated N-glycan

KW - Tamm-Horsfall glycoprotein

KW - Tamm Horsfall glycoprotein

KW - article

KW - carbohydrate analysis

KW - controlled study

KW - drug isolation

KW - drug structure

KW - high performance liquid chromatography

KW - human

KW - human tissue

KW - normal human

KW - nuclear magnetic resonance spectroscopy

KW - priority journal

KW - urine

U2 - 10.1046/j.1432-1327.1998.2560471.x

DO - 10.1046/j.1432-1327.1998.2560471.x

M3 - Article

SN - 0014-2956

VL - 256

SP - 471

EP - 487

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

IS - 2

ER -

Sulfated di-, tri- and tetraantennary N-glycans in human Tamm-Horsfall glycoprotein

Abstract

UN SDGs

Keywords

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