Suitability of synchrotron-radiation phase-contrast tomography for imaging of inner ear of harbor porpoise

Laura Rojas*, Julian Moosmann, Felix Beckmann, Louise Moysan, Ursula Siebert, Lonneke IJsseldijk, Imke A.K. Fiedler, Björn Busse, Maria Morell

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Synchrotron-radiation phase-contrast tomography is a non-destructive technique that allows high-resolution imaging of soft structures. We present the first results of the cochlea (inner ear) of a harbor porpoise (Phocoena phocoena). Samples were analyzed at a high spatial resolution using synchrotron-radiation propagation-based phase-contrast microtomography at the high-energy materials science beamline (HEMS) P07, operated by Hereon, at the storage ring PETRA III at DESY. Since these samples are larger than the X-ray beam profile, a scanning and projection-based stitching approach was used. Stitching of the acquired raw data sets resulted in centimeter-sized reconstructions with 2.54 µm voxel size and volumes ranging from 280 GB to 5.2 TB. Synchrotron-radiation phase-contrast tomography proved to be a suitable technique to image the spiral ganglion cells and other structures of the inner ear of a harbor porpoise.

Original languageEnglish
Title of host publicationDevelopments in X-Ray Tomography XV
EditorsBert Muller, Ge Wang
PublisherSPIE
ISBN (Electronic)9781510679641
DOIs
Publication statusPublished - 2024
Event15th SPIE Conference on Developments in X-Ray Tomography - San Diego, United States
Duration: 19 Aug 202422 Aug 2024

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume13152
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

Conference15th SPIE Conference on Developments in X-Ray Tomography
Country/TerritoryUnited States
CitySan Diego
Period19/08/2422/08/24

Keywords

  • cochlea
  • harbor porpoise
  • inner ear
  • phase contrast
  • stitching
  • tomography
  • X-ray imaging

Fingerprint

Dive into the research topics of 'Suitability of synchrotron-radiation phase-contrast tomography for imaging of inner ear of harbor porpoise'. Together they form a unique fingerprint.

Cite this