Abstract
Sulphated N-linked carbohydrate chains isolated from recombinant human tissue plasminogen activator expressed in mouse epithelial (C127) cells were analysed as oligosaccharide alditols by methylation analysis, liquid secondary ion mass spectrometry, and one- and two-dimensional 1H-NMR spectroscopy. The results demonstrate that the major component has the following novel structure: NeuAcα2-6 Galβ1-4GlcNAcβ1-2[NeuAcα2-3Galβ1-4GlcNAcβ1-4]Manα1-3[NeuAcα2- 3( SO4-6)Galβ1-4GlcNAcβ1-2Manα1-6]Manβ1-4GlcNAcβ1-4[Fucα1-6]GlcN Ac -ol.
Original language | English |
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Pages (from-to) | 411-418 |
Number of pages | 8 |
Journal | Glycobiology |
Volume | 2 |
Issue number | 5 |
Publication status | Published - 12 Jul 1992 |
Keywords
- oligosaccharide sulfate
- recombinant protein
- tissue plasminogen activator
- unclassified drug
- animal cell
- article
- chemical structure
- epithelium cell
- human
- mass spectrometry
- mouse
- nonhuman
- proton nuclear magnetic resonance