Structure elucidation of sulphated oligosaccharides from recombinant human tissue plasminogen activator expressed in mouse epithelial cells

G. Pfeiffer, S. Stirm, R. Geyer, K.-H. Strube, A.A. Bergwerff, J.P. Kamerling, J.F.G. Vliegenthart

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Sulphated N-linked carbohydrate chains isolated from recombinant human tissue plasminogen activator expressed in mouse epithelial (C127) cells were analysed as oligosaccharide alditols by methylation analysis, liquid secondary ion mass spectrometry, and one- and two-dimensional 1H-NMR spectroscopy. The results demonstrate that the major component has the following novel structure: NeuAcα2-6 Galβ1-4GlcNAcβ1-2[NeuAcα2-3Galβ1-4GlcNAcβ1-4]Manα1-3[NeuAcα2- 3( SO4-6)Galβ1-4GlcNAcβ1-2Manα1-6]Manβ1-4GlcNAcβ1-4[Fucα1-6]GlcN Ac -ol.
Original languageEnglish
Pages (from-to)411-418
Number of pages8
JournalGlycobiology
Volume2
Issue number5
Publication statusPublished - 12 Jul 1992

Keywords

  • oligosaccharide sulfate
  • recombinant protein
  • tissue plasminogen activator
  • unclassified drug
  • animal cell
  • article
  • chemical structure
  • epithelium cell
  • human
  • mass spectrometry
  • mouse
  • nonhuman
  • proton nuclear magnetic resonance

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