Structural studies on exopolysaccharides produced by three different propionibacteria strains

J.M. Dobruchowska; G.J. Gerwig; A. Babuchowski; J.P. Kamerling

Structural studies on exopolysaccharides produced by three different propionibacteria strains

J.M. Dobruchowska, G.J. Gerwig, A. Babuchowski, J.P. Kamerling

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Abstract

The exopolysaccharides produced by three propionibacteria strains, Propionibacterium freudenreichii 109, Propionibacterium freudenreichii 111, and Propionibacterium thoenii 126, grown on whey-based media, were found to be charged heteropolymers, composed of D-glucose, D-mannose, and D-glucuronic acid in molar ratios of 2:2:1. By means of methylation analysis, mass spectrometry, partial acid hydrolysis, and 1D/2D NMR (1H and 13C) studies, it was determined that all three exopolysaccharides contain the same branched, pentasaccharide repeating unit: β-D-GlcpA α-D-Glcp 1 1 ↓ ↓ 6 6 →3) -α-D-Manp-(1→3) -α-D-Manp-(1→3) -α-D-Glcp-(1→

Original language	Undefined/Unknown
Pages (from-to)	726-745
Number of pages	21
Journal	Carbohydrate Research
Volume	343
Publication status	Published - 2008

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title = "Structural studies on exopolysaccharides produced by three different propionibacteria strains",

abstract = "The exopolysaccharides produced by three propionibacteria strains, Propionibacterium freudenreichii 109, Propionibacterium freudenreichii 111, and Propionibacterium thoenii 126, grown on whey-based media, were found to be charged heteropolymers, composed of D-glucose, D-mannose, and D-glucuronic acid in molar ratios of 2:2:1. By means of methylation analysis, mass spectrometry, partial acid hydrolysis, and 1D/2D NMR (1H and 13C) studies, it was determined that all three exopolysaccharides contain the same branched, pentasaccharide repeating unit: β-D-GlcpA α-D-Glcp 1 1 ↓ ↓ 6 6 →3) -α-D-Manp-(1→3) -α-D-Manp-(1→3) -α-D-Glcp-(1→",

author = "J.M. Dobruchowska and G.J. Gerwig and A. Babuchowski and J.P. Kamerling",

year = "2008",

language = "Undefined/Unknown",

volume = "343",

pages = "726--745",

journal = "Carbohydrate Research",

issn = "0008-6215",

publisher = "Elsevier Ltd",

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TY - JOUR

T1 - Structural studies on exopolysaccharides produced by three different propionibacteria strains

AU - Dobruchowska, J.M.

AU - Gerwig, G.J.

AU - Babuchowski, A.

AU - Kamerling, J.P.

PY - 2008

Y1 - 2008

N2 - The exopolysaccharides produced by three propionibacteria strains, Propionibacterium freudenreichii 109, Propionibacterium freudenreichii 111, and Propionibacterium thoenii 126, grown on whey-based media, were found to be charged heteropolymers, composed of D-glucose, D-mannose, and D-glucuronic acid in molar ratios of 2:2:1. By means of methylation analysis, mass spectrometry, partial acid hydrolysis, and 1D/2D NMR (1H and 13C) studies, it was determined that all three exopolysaccharides contain the same branched, pentasaccharide repeating unit: β-D-GlcpA α-D-Glcp 1 1 ↓ ↓ 6 6 →3) -α-D-Manp-(1→3) -α-D-Manp-(1→3) -α-D-Glcp-(1→

AB - The exopolysaccharides produced by three propionibacteria strains, Propionibacterium freudenreichii 109, Propionibacterium freudenreichii 111, and Propionibacterium thoenii 126, grown on whey-based media, were found to be charged heteropolymers, composed of D-glucose, D-mannose, and D-glucuronic acid in molar ratios of 2:2:1. By means of methylation analysis, mass spectrometry, partial acid hydrolysis, and 1D/2D NMR (1H and 13C) studies, it was determined that all three exopolysaccharides contain the same branched, pentasaccharide repeating unit: β-D-GlcpA α-D-Glcp 1 1 ↓ ↓ 6 6 →3) -α-D-Manp-(1→3) -α-D-Manp-(1→3) -α-D-Glcp-(1→

M3 - Article

SN - 0008-6215

VL - 343

SP - 726

EP - 745

JO - Carbohydrate Research

JF - Carbohydrate Research

ER -