Abstract
The POU-domain transcription factor Pit-1 and Ets-1, a member of
the ETS family of transcription factors, can associate in solution and
synergistically activate the prolactin promoter by binding to a
composite response element in the prolactin promoter. We
mapped the minimal region of Ets-1 required for the interaction
with the Pit-1 POU-homeodomain. Here, we describe a detailed
NMR study of the interaction between the POU-homeodomain of
Pit-1 and the minimal interacting region of Ets-1. By using heteronuclear
single quantum coherence titration experiments, we were
able to map exact residues on the POU-homeodomain that are
involved in the interaction with this minimal Ets-1 interaction
domain. By using ourNMRdata, we generated point mutants in the
POU-homeodomain and tested their effect on the interaction with
Ets-1. Our results show that phosphorylation of Pit-1 can regulate
the interaction with Ets-1.
| Original language | Undefined/Unknown |
|---|---|
| Pages (from-to) | 12657-12662 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 99 |
| Issue number | 20 |
| Publication status | Published - 2002 |