Structural and Functional Characterization of the Newly Designed Antimicrobial Peptide Crabrolin21

Francesca Cantini; Paola Giannì; Sara Bobone; Cassandra Troiano; Hugo van Ingen; Renato Massoud; Nicoletta Perini; Luciana Migliore; Philippe Savarin; Charles Sanders; Lorenzo Stella; Marco Sette

doi:10.3390/membranes13030365

Structural and Functional Characterization of the Newly Designed Antimicrobial Peptide Crabrolin21

Francesca Cantini
, Paola Giannì
, Sara Bobone
, Cassandra Troiano
, Hugo van Ingen
, Renato Massoud
, Nicoletta Perini
, Luciana Migliore
, Philippe Savarin
, Charles Sanders
, Lorenzo Stella
, Marco Sette^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

(1) Background: antimicrobial resistance is becoming a dramatic problem for public health, and the design of new antimicrobial agents is an active research area. (2) Methods: based on our previous work, we designed an improved version of the crabrolin peptide and characterized its functional and structural properties with a wide range of techniques. (3) Results: the newly designed peptide, crabrolin21, is much more active than the previous ones and shows specific selectivity towards bacterial cells. (4) Conclusions: crabrolin21 shows interesting properties and deserves further studies.

Original language	English
Article number	365
Pages (from-to)	1-13
Number of pages	13
Journal	Membranes
Volume	13
Issue number	3
DOIs	https://doi.org/10.3390/membranes13030365
Publication status	Published - Mar 2023

Bibliographical note

Publisher Copyright:
© 2023 by the authors.

Funding

This work benefited from access to the Utrecht NMR Facility and was supported by iNEXT-Discovery, project number 871037, funded by the Horizon 2020 program of the European Commission, the PRIN project 2020833Y75 (Italian Ministry of University and Research) to L.S. and M.S., and partial support from the Regione Lazio ‘Contributo premiale per i ricercatori 2022’, DE G05411.

Funders	Funder number
iNEXT-Discovery - Horizon 2020 program of the European Commission	871037
PRIN project (Italian Ministry of University and Research)	2020833Y75
Regione Lazio Contributo premiale per i ricercatori 2022'	DE G05411

Keywords

antimicrobial resistance
CD spectroscopy
membrane specificity
NMR spectroscopy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/membranes13030365Licence: CC BY

membranes-13-00365Final published version, 2.02 MBLicence: CC BY

Cite this

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abstract = "(1) Background: antimicrobial resistance is becoming a dramatic problem for public health, and the design of new antimicrobial agents is an active research area. (2) Methods: based on our previous work, we designed an improved version of the crabrolin peptide and characterized its functional and structural properties with a wide range of techniques. (3) Results: the newly designed peptide, crabrolin21, is much more active than the previous ones and shows specific selectivity towards bacterial cells. (4) Conclusions: crabrolin21 shows interesting properties and deserves further studies.",

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T1 - Structural and Functional Characterization of the Newly Designed Antimicrobial Peptide Crabrolin21

AU - Cantini, Francesca

AU - Giannì, Paola

AU - Bobone, Sara

AU - Troiano, Cassandra

AU - van Ingen, Hugo

AU - Massoud, Renato

AU - Perini, Nicoletta

AU - Migliore, Luciana

AU - Savarin, Philippe

AU - Sanders, Charles

AU - Stella, Lorenzo

AU - Sette, Marco

PY - 2023/3

Y1 - 2023/3

N2 - (1) Background: antimicrobial resistance is becoming a dramatic problem for public health, and the design of new antimicrobial agents is an active research area. (2) Methods: based on our previous work, we designed an improved version of the crabrolin peptide and characterized its functional and structural properties with a wide range of techniques. (3) Results: the newly designed peptide, crabrolin21, is much more active than the previous ones and shows specific selectivity towards bacterial cells. (4) Conclusions: crabrolin21 shows interesting properties and deserves further studies.

AB - (1) Background: antimicrobial resistance is becoming a dramatic problem for public health, and the design of new antimicrobial agents is an active research area. (2) Methods: based on our previous work, we designed an improved version of the crabrolin peptide and characterized its functional and structural properties with a wide range of techniques. (3) Results: the newly designed peptide, crabrolin21, is much more active than the previous ones and shows specific selectivity towards bacterial cells. (4) Conclusions: crabrolin21 shows interesting properties and deserves further studies.

KW - antimicrobial resistance

KW - CD spectroscopy

KW - membrane specificity

KW - NMR spectroscopy

UR - https://www.scopus.com/pages/publications/85152214437

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DO - 10.3390/membranes13030365

M3 - Article

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ER -

Structural and Functional Characterization of the Newly Designed Antimicrobial Peptide Crabrolin21

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

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