Structural analysis of underivatized neutral human milk oligosaccharides in the negative ion mode by nano-electrospray MSn (Part 2: Application to isomeric mixtures)

Anja Pfenninger, Michael Karas*, Berndt Finke, Bernd Stahl

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

A complex mixture of isomeric neutral oligosaccharides from pooled human milk was analyzed by nano-electrospray ionization (ESI) in a quadrupole ion trap mass spectrometer (QIT-MS) in the negative ion mode. Since deprotonated molecules of neutral oligosaccharides follow distinct fragmentation rules, which have been elucidated by using model compounds (see [1]), spectra obtained from consecutive CID experiments allowed the differentiation of isomers out of this highly complex mixture. With this method new human milk oligosaccharides of previously unknown isomeric structures have been identified, e.g., the occurence of three isomeric fucosylated lacto-N-hexaoses could be determined precisely, which have not been described before: (1) Fuc (α1 → 2) Gal (β1 → 3) GlcNac (β1 → 3) Gal (β1 → 4) GlcNac (β1 → 3) Gal (β1 → 4) Glc, (2) Gal (β1 → 4) GlcNAc [(α1 → 3) Fuc] (β1 → 3) Gal (β1 → 4) GlcNac (β1 → 3) Gal (β1 → 4) Glc, (3) Gal (β1 → 4) GlcNAc (β1 → 3) Gal (β1 → 4) GlcNac [(α1 → 3) Fuc] (β1 → 3) Gal (β1 → 4) Glc.

Original languageEnglish
Pages (from-to)1341-1348
Number of pages8
JournalJournal of the American Society for Mass Spectrometry
Volume13
Issue number11
DOIs
Publication statusPublished - Nov 2002
Externally publishedYes

Bibliographical note

Funding Information:
The authors thank Dr. Hildegard Geyer and Dr. U. Bahr for helpful discussions. They also gratefully acknowledge financial support by the Bundesministerium für Bildung und Forschung (BMBF) under grant BEO/22 0311229.

Funding

The authors thank Dr. Hildegard Geyer and Dr. U. Bahr for helpful discussions. They also gratefully acknowledge financial support by the Bundesministerium für Bildung und Forschung (BMBF) under grant BEO/22 0311229.

Fingerprint

Dive into the research topics of 'Structural analysis of underivatized neutral human milk oligosaccharides in the negative ion mode by nano-electrospray MSn (Part 2: Application to isomeric mixtures)'. Together they form a unique fingerprint.

Cite this