Structural analysis of the α--D-glucan (EPS35-5) produced by the Lactobacillus reuteri strain 35-5 glucansucrase GTFA enzyme

S.S. van Leeuwen; S. Kralj; I.H. van Geel-Schutten; G.J. Gerwig; L. Dijkhuizen; J.P. Kamerling

Structural analysis of the α--D-glucan (EPS35-5) produced by the Lactobacillus reuteri strain 35-5 glucansucrase GTFA enzyme

S.S. van Leeuwen, S. Kralj, I.H. van Geel-Schutten, G.J. Gerwig, L. Dijkhuizen, J.P. Kamerling

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Abstract

The neutral exopolysaccharide EPS35-5 (reuteran) produced from sucrose by the glucansucrase GTFA enzyme from Lactobacillus reuteri 35-5 was found to be a (1?4,1?6)-α-D-glucan, with no repeating units present. Based on linkage analysis and 1D/2D 1H and 13C NMR spectroscopy of intact EPS35-5, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis and enzymatic hydrolysis, using pullulanase M1 (Klebsiella planticola), of EPS35-5, a composite model, that includes all identified structural elements, was formulated as follows:

Original language	Undefined/Unknown
Pages (from-to)	1251-1265
Number of pages	15
Journal	Carbohydrate Research
Volume	343
Issue number	7
Publication status	Published - 2008

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@article{ff61063386874181b7d6dd62fa285f3d,

title = "Structural analysis of the α--D-glucan (EPS35-5) produced by the Lactobacillus reuteri strain 35-5 glucansucrase GTFA enzyme",

abstract = "The neutral exopolysaccharide EPS35-5 (reuteran) produced from sucrose by the glucansucrase GTFA enzyme from Lactobacillus reuteri 35-5 was found to be a (1?4,1?6)-α-D-glucan, with no repeating units present. Based on linkage analysis and 1D/2D 1H and 13C NMR spectroscopy of intact EPS35-5, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis and enzymatic hydrolysis, using pullulanase M1 (Klebsiella planticola), of EPS35-5, a composite model, that includes all identified structural elements, was formulated as follows:",

author = "{van Leeuwen}, S.S. and S. Kralj and {van Geel-Schutten}, I.H. and G.J. Gerwig and L. Dijkhuizen and J.P. Kamerling",

year = "2008",

language = "Undefined/Unknown",

volume = "343",

pages = "1251--1265",

journal = "Carbohydrate Research",

issn = "0008-6215",

publisher = "Elsevier BV",

number = "7",

}

TY - JOUR

T1 - Structural analysis of the α--D-glucan (EPS35-5) produced by the Lactobacillus reuteri strain 35-5 glucansucrase GTFA enzyme

AU - van Leeuwen, S.S.

AU - Kralj, S.

AU - van Geel-Schutten, I.H.

AU - Gerwig, G.J.

AU - Dijkhuizen, L.

AU - Kamerling, J.P.

PY - 2008

Y1 - 2008

N2 - The neutral exopolysaccharide EPS35-5 (reuteran) produced from sucrose by the glucansucrase GTFA enzyme from Lactobacillus reuteri 35-5 was found to be a (1?4,1?6)-α-D-glucan, with no repeating units present. Based on linkage analysis and 1D/2D 1H and 13C NMR spectroscopy of intact EPS35-5, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis and enzymatic hydrolysis, using pullulanase M1 (Klebsiella planticola), of EPS35-5, a composite model, that includes all identified structural elements, was formulated as follows:

AB - The neutral exopolysaccharide EPS35-5 (reuteran) produced from sucrose by the glucansucrase GTFA enzyme from Lactobacillus reuteri 35-5 was found to be a (1?4,1?6)-α-D-glucan, with no repeating units present. Based on linkage analysis and 1D/2D 1H and 13C NMR spectroscopy of intact EPS35-5, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis and enzymatic hydrolysis, using pullulanase M1 (Klebsiella planticola), of EPS35-5, a composite model, that includes all identified structural elements, was formulated as follows:

M3 - Article

SN - 0008-6215

VL - 343

SP - 1251

EP - 1265

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 7

ER -